Detailed information of Recipient susceptibility factor


Summary

ID RSF000059 experimental
Strain Klebsiella pneumoniae strain SB617
Replicon chromosome
Sequence Protein sequence (379 a.a.); Nucleotide sequence (1137 bp)
Response to T6SS attack protective
Known or putative function capsule protection
Description Group I capsules confer a strong protection from V. cholerae's T6SS attacks to commensal Klebsiella strains.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) LGM18_RS08295 (wza)
Coordinates (Strand) 1719795..1720931 (+)
NCBI ID WP_136085646.1
RefSeq NZ_CP084825
Uniprot ID UPI00109D275D
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Poly_export PF02563.18 6.8e-28 82..165
Wza_C PF18412.3 3.7e-18 345..374
SLBB PF10531.11 5.2e-08 173..217
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MKKK
Transmembrane helix 5-27 IVRFSALALAIGFLSGCTIIPGQ
Outside 28-378 GLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQVDVSVAAFRSQKAYVTGEVAKSGQQAITNIPLTVMDAINAAGGLAEDADWRNVVLTHNGKDKKISLYALMQKGDLTQNELLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEALGNAEGMSQAFSDATGVFVIRQLKGDSKGKIANIYQLNAQDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETARYIKSWP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9982 pos: 20-21. LSG-CT. MKKKIVRFSALALAIGFLSG


  Protein sequence: 379 a.a.    .

>RSF000059 NZ_CP084825:1719795-1720931 [Klebsiella pneumoniae]
MKKKIVRFSALALAIGFLSGCTIIPGQGLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLL
RSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQ
VDVSVAAFRSQKAYVTGEVAKSGQQAITNIPLTVMDAINAAGGLAEDADWRNVVLTHNGKDKKISLYALMQKGDLTQNEL
LYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEALGNAEGMSQAFSDATGVFVIRQLKGDSKGKIANIYQLNA
QDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETARYIKSWP*

  Nucleotide sequence: 1137 bp    .

>RSF000059 NZ_CP084825:1719795-1720931 [Klebsiella pneumoniae]
ATGAAGAAAAAAATTGTTAGATTTTCGGCATTAGCGTTGGCAATTGGGTTTTTATCGGGTTGTACCATTATCCCTGGTCA
GGGATTAAACAGTCTGCGTAAGAACGTGGTTGAACTCCCAGATAGTGATTACGATTTGGATAAGTTGGTCAATGTATATC
CGATGACACCAGGATTGATTGATCAACTTCGCCCAGAAACCGTTCTGGCTCGTCCCAATCCGCAACTGGATAACTTACTC
CGTAGTTATGAATATCGCATTGGGGTAGGTGATGTACTGATGGTAACCGTTTGGGATCACCCGGAACTGACAACACCAGC
AGGTCAGTACCGTAGCGCCAGCGACACTGGTAACTGGGTTAACTCTGACGGTACCATTTTCTATCCATATATTGGTAAGG
TGCAGGTGGCGGGCAAAACGCTTAGCCAGGTACGCCAGGATATAGCCAGTCGTTTAACCACCTATATTGAAAGTCCCCAA
GTAGATGTTAGCGTTGCTGCATTTCGTTCGCAAAAAGCATATGTAACGGGTGAGGTTGCAAAATCGGGACAACAGGCGAT
AACTAACATTCCACTCACTGTCATGGATGCGATAAATGCAGCAGGAGGGCTGGCAGAGGATGCTGACTGGCGTAATGTAG
TCTTGACGCACAATGGTAAAGACAAAAAGATATCTTTATATGCCCTTATGCAAAAAGGTGACTTGACGCAGAATGAGTTG
CTTTATCCAGGTGATATTTTATTTGTGCCACGTAATGATGACCTTAAAGTATTTGTCATGGGTGAGGTCGGCAAGCAAAG
CACTATGAAGATGGATCGAAGTGGAATGACACTTGCAGAAGCCCTTGGAAATGCTGAAGGGATGTCACAAGCATTTAGCG
ATGCGACAGGGGTTTTCGTAATTCGACAGTTAAAAGGCGACAGCAAGGGAAAGATTGCTAATATATATCAGCTTAATGCA
CAGGATGCGTCAGCGATGGTGTTAGGTACAGAATTCCAATTGCAGCCCTATGATATTGTTTATGTGACGACAGCACCTTT
GGTTCGTTGGAATCGTGTCATATCACAGCTGGTGCCTACGATAACAGGCGTACATGATATGACAGAAACTGCCCGTTATA
TTAAATCTTGGCCATAA