Detailed information of effector
Summary
| Effector ID | EFF01810  |
| Effector type | Tde |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00551 (Type i3) |
| Strain | Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S |
| Replicon | chromosome |
| Sequence | Protein sequence (174 a.a.); Nucleotide sequence (522 bp) |
| Description | Tlde1 is an anti-bacterial effector secreted via the SPI-6 T6SS and Tlde1 Intoxication causes altered cell division, swelling, and lysis. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | STM14_0336 (Tlde1) |
| Coordinates (Strand) | 329640..330161 (+) |
| NCBI ID | 446891128 |
| RefSeq | NC_016856 |
| Uniprot ID | V7IRB5_SALET, M7RFX4_SALDU, A0A2R4HUU4_SALEN, A0A2T8L422_SALET, A0A0H3ND49_SALTS, A0A232R996_SALER, Q57SX4_SALCH, A0A2T9Q8Y9_SALET, A0A2R4DD94_SALET, A0A2T8MP53_SALAN, A0A0F6AX90_SALT1, A0A2T6X0K3_SALET, A0A265B9N8_SALET, H9L4J5_SALTM, E8XIV3_SALT4, Q93IR4_SALTY |
| KEGG ID | seo:STM14_0336, sey:SL1344_0283, seb:STM474_0300, sec:SCH_0281, stm:STM0288 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF2778 | PF10908.10 | 1.7e-05 | 34..151 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-173 | MMAVRLTFDGQKLTWPGIGIFKATTGLPDLQWPDKQCVPDAAIPEGNYKLFIQFQGEAPIRNAADCDLGPSWGWSTIPRGQAAGTCEIYWANWGYNRIRLESADEKTRKACGGKRGGFYIHDSTKGYSHGCIEVEPVFFRILKQETEKENGEKTFTVNVKYVSGQQTNGGTKQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9595 | - | - |
Protein sequence: 174 a.a. .
>EFF01810 NC_016856:329640-330161 Type VI DNase and RNase Effector (Tde) [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
MMAVRLTFDGQKLTWPGIGIFKATTGLPDLQWPDKQCVPDAAIPEGNYKLFIQFQGEAPIRNAADCDLGPSWGWSTIPRG
QAAGTCEIYWANWGYNRIRLESADEKTRKACGGKRGGFYIHDSTKGYSHGCIEVEPVFFRILKQETEKENGEKTFTVNVK
YVSGQQTNGGTKQ*
MMAVRLTFDGQKLTWPGIGIFKATTGLPDLQWPDKQCVPDAAIPEGNYKLFIQFQGEAPIRNAADCDLGPSWGWSTIPRG
QAAGTCEIYWANWGYNRIRLESADEKTRKACGGKRGGFYIHDSTKGYSHGCIEVEPVFFRILKQETEKENGEKTFTVNVK
YVSGQQTNGGTKQ*
Nucleotide sequence: 522 bp .
>EFF01810 NC_016856:329640-330161 Type VI DNase and RNase Effector (Tde) [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
ATGATGGCAGTAAGACTGACTTTTGACGGGCAAAAGCTGACATGGCCTGGTATCGGGATATTTAAGGCGACCACGGGGTT
ACCGGATTTACAGTGGCCAGATAAACAGTGTGTGCCGGATGCGGCGATACCGGAAGGGAATTATAAATTGTTTATTCAGT
TTCAGGGGGAGGCACCGATAAGAAATGCTGCGGATTGTGATCTGGGACCATCATGGGGCTGGAGTACCATTCCGCGAGGC
CAGGCTGCCGGAACATGTGAGATATACTGGGCGAACTGGGGATATAATCGTATCCGGCTGGAATCAGCGGATGAGAAGAC
CCGAAAAGCCTGTGGGGGCAAGCGGGGTGGTTTTTATATCCATGATTCCACCAAAGGTTACAGTCATGGTTGTATTGAAG
TGGAACCGGTGTTTTTCCGTATTCTGAAACAGGAGACGGAAAAAGAAAATGGTGAAAAGACATTTACGGTTAATGTTAAG
TATGTTTCTGGTCAGCAAACGAATGGTGGAACAAAACAATAA
ATGATGGCAGTAAGACTGACTTTTGACGGGCAAAAGCTGACATGGCCTGGTATCGGGATATTTAAGGCGACCACGGGGTT
ACCGGATTTACAGTGGCCAGATAAACAGTGTGTGCCGGATGCGGCGATACCGGAAGGGAATTATAAATTGTTTATTCAGT
TTCAGGGGGAGGCACCGATAAGAAATGCTGCGGATTGTGATCTGGGACCATCATGGGGCTGGAGTACCATTCCGCGAGGC
CAGGCTGCCGGAACATGTGAGATATACTGGGCGAACTGGGGATATAATCGTATCCGGCTGGAATCAGCGGATGAGAAGAC
CCGAAAAGCCTGTGGGGGCAAGCGGGGTGGTTTTTATATCCATGATTCCACCAAAGGTTACAGTCATGGTTGTATTGAAG
TGGAACCGGTGTTTTTCCGTATTCTGAAACAGGAGACGGAAAAAGAAAATGGTGAAAAGACATTTACGGTTAATGTTAAG
TATGTTTCTGGTCAGCAAACGAATGGTGGAACAAAACAATAA