285017

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1533875..1534401 Replicon chromosome
Accession NZ_HG738867
Organism Escherichia coli str. K-12 substr. MC4100 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BN896_RS07645 Protein ID WP_000323025.1
Coordinates 1533875..1534162 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BN896_RS07650 Protein ID WP_000534858.1
Coordinates 1534162..1534401 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN896_RS07605 (BN896_1375) 1528899..1529114 - 216 WP_000839590.1 phage lysis protein EssD -
BN896_RS07610 (BN896_1376) 1529868..1530083 - 216 WP_000066484.1 cold shock-like protein CspB -
BN896_RS07615 (BN896_1377) 1530384..1530596 + 213 WP_000087756.1 cold shock-like protein CspF -
BN896_RS25110 1530651..1530740 + 90 WP_120795389.1 hypothetical protein -
BN896_RS07620 (BN896_1378) 1531018..1531770 - 753 WP_001047135.1 antitermination protein -
BN896_RS07625 (BN896_1379) 1531784..1532833 - 1050 WP_001265198.1 DUF968 domain-containing protein -
BN896_RS07630 1532835..1533113 - 279 WP_012304870.1 hypothetical protein -
BN896_RS07635 (BN896_1380) 1533180..1533431 - 252 WP_000980994.1 hypothetical protein -
BN896_RS07640 (BN896_1381) 1533648..1533803 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
BN896_RS07645 (BN896_1382) 1533875..1534162 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BN896_RS07650 (BN896_1383) 1534162..1534401 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BN896_RS23255 (BN896_1384) 1534426..1534731 + 306 WP_001326990.1 hypothetical protein -
BN896_RS07655 (BN896_1385) 1534934..1535266 + 333 WP_001301033.1 protein FlxA -
BN896_RS22595 (BN896_1386) 1535703..1535852 - 150 WP_011443592.1 hypothetical protein -
BN896_RS07660 (BN896_1387) 1535887..1536165 - 279 Protein_1460 hypothetical protein -
BN896_RS07665 (BN896_1388) 1536149..1536379 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
BN896_RS07670 (BN896_1389) 1536463..1536870 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
BN896_RS07675 (BN896_1390) 1537037..1537192 + 156 WP_000379575.1 DUF1391 family protein -
BN896_RS07680 1537352..1537570 + 219 WP_001171942.1 hypothetical protein -
BN896_RS07690 (BN896_1392) 1538138..1538326 + 189 WP_000854559.1 cell division inhibition protein DicB -
BN896_RS07695 (BN896_1393) 1538323..1538514 + 192 WP_001083297.1 lysis protein YdfD -
BN896_RS23270 1538607..1539374 + 768 Protein_1467 exonuclease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1519531..1552992 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T285017 WP_000323025.1 NZ_HG738867:c1534162-1533875 [Escherichia coli str. K-12 substr. MC4100]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T285017 NZ_HG738867:c1534162-1533875 [Escherichia coli str. K-12 substr. MC4100]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT285017 WP_000534858.1 NZ_HG738867:c1534401-1534162 [Escherichia coli str. K-12 substr. MC4100]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT285017 NZ_HG738867:c1534401-1534162 [Escherichia coli str. K-12 substr. MC4100]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References