Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639603..1640129 Replicon chromosome
Accession NZ_CP064682
Organism Escherichia coli K-12 strain BZKR2G40

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IVK99_RS08025 Protein ID WP_000323025.1
Coordinates 1639603..1639890 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IVK99_RS08030 Protein ID WP_000534858.1
Coordinates 1639890..1640129 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVK99_RS07980 1634627..1634842 - 216 WP_000839590.1 phage lysis protein EssD -
IVK99_RS07985 1635596..1635811 - 216 WP_000066484.1 cold shock-like protein CspB -
IVK99_RS07990 1636112..1636324 + 213 WP_000087756.1 cold shock-like protein CspF -
IVK99_RS07995 1636379..1636468 + 90 WP_120795389.1 hypothetical protein -
IVK99_RS08000 1636746..1637498 - 753 WP_001047135.1 antitermination protein -
IVK99_RS08005 1637512..1638561 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IVK99_RS08010 1638563..1638841 - 279 WP_012304870.1 hypothetical protein -
IVK99_RS08015 1638908..1639159 - 252 WP_000980994.1 hypothetical protein -
IVK99_RS08020 1639376..1639531 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IVK99_RS08025 1639603..1639890 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IVK99_RS08030 1639890..1640129 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IVK99_RS08035 1640154..1640459 + 306 WP_001326990.1 hypothetical protein -
IVK99_RS08040 1640662..1640994 + 333 WP_001301033.1 protein FlxA -
IVK99_RS08045 1641431..1641580 - 150 WP_011443592.1 hypothetical protein -
IVK99_RS08050 1641615..1641893 - 279 Protein_1573 hypothetical protein -
IVK99_RS08055 1641877..1642107 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IVK99_RS08060 1642191..1642598 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IVK99_RS08065 1642765..1642920 + 156 WP_000379575.1 DUF1391 family protein -
IVK99_RS08070 1643080..1643298 + 219 WP_001171942.1 hypothetical protein -
IVK99_RS08075 1643866..1644054 + 189 WP_000854559.1 cell division inhibition protein DicB -
IVK99_RS08080 1644051..1644242 + 192 WP_001083297.1 lysis protein YdfD -
IVK99_RS08085 1644335..1645102 + 768 Protein_1580 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625259..1658720 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T181599 WP_000323025.1 NZ_CP064682:c1639890-1639603 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T181599 NZ_CP085981:c3464869-3464558 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT181599 WP_000534858.1 NZ_CP064682:c1640129-1639890 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT181599 NZ_CP085981:c3464561-3464115 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References