22686

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1732995..1733521 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECDH10B_RS08690 Protein ID WP_000323025.1
Coordinates 1732995..1733282 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECDH10B_RS08695 Protein ID WP_000534858.1
Coordinates 1733282..1733521 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS08650 (ECDH10B_1688) 1728019..1728234 - 216 WP_000839590.1 phage lysis protein EssD -
ECDH10B_RS08655 (ECDH10B_1689) 1728988..1729203 - 216 WP_000066484.1 cold shock-like protein CspB -
ECDH10B_RS08660 (ECDH10B_1690) 1729504..1729716 + 213 WP_000087756.1 cold shock-like protein CspF -
ECDH10B_RS26005 1729771..1729860 + 90 WP_120795389.1 hypothetical protein -
ECDH10B_RS08665 (ECDH10B_1691) 1730138..1730890 - 753 WP_001047135.1 antitermination protein -
ECDH10B_RS08670 1730904..1731953 - 1050 WP_001265198.1 DUF968 domain-containing protein -
ECDH10B_RS08675 1731955..1732233 - 279 WP_012304870.1 hypothetical protein -
ECDH10B_RS08680 (ECDH10B_1693) 1732300..1732551 - 252 WP_000980994.1 hypothetical protein -
ECDH10B_RS08685 (ECDH10B_1694) 1732768..1732923 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ECDH10B_RS08690 (ECDH10B_1695) 1732995..1733282 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECDH10B_RS08695 (ECDH10B_1696) 1733282..1733521 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECDH10B_RS24165 (ECDH10B_1697) 1733546..1733851 + 306 WP_001326990.1 hypothetical protein -
ECDH10B_RS08700 (ECDH10B_1698) 1734054..1734386 + 333 WP_001301033.1 protein FlxA -
ECDH10B_RS23370 (ECDH10B_1699) 1734823..1734972 - 150 WP_011443592.1 hypothetical protein -
ECDH10B_RS08705 (ECDH10B_1700) 1735007..1735285 - 279 Protein_1666 hypothetical protein -
ECDH10B_RS08710 (ECDH10B_1701) 1735269..1735499 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
ECDH10B_RS08715 (ECDH10B_1702) 1735583..1735990 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ECDH10B_RS08720 (ECDH10B_1703) 1736157..1736312 + 156 WP_000379575.1 DUF1391 family protein -
ECDH10B_RS08725 (ECDH10B_1705) 1736472..1736690 + 219 WP_001171942.1 hypothetical protein -
ECDH10B_RS08735 (ECDH10B_1707) 1737258..1737446 + 189 WP_000854559.1 cell division inhibition protein DicB -
ECDH10B_RS08740 (ECDH10B_1708) 1737443..1737634 + 192 WP_001083297.1 lysis protein YdfD -
ECDH10B_RS24180 (ECDH10B_1709) 1737727..1738494 + 768 Protein_1673 exonuclease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1718651..1753448 34797


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T22686 WP_000323025.1 NC_010473:c1733282-1732995 [Escherichia coli str. K-12 substr. DH10B]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T22686 NC_010473:c1733282-1732995 [Escherichia coli str. K-12 substr. DH10B]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT22686 WP_000534858.1 NC_010473:c1733521-1733282 [Escherichia coli str. K-12 substr. DH10B]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT22686 NC_010473:c1733521-1733282 [Escherichia coli str. K-12 substr. DH10B]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References