Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639599..1640125 Replicon chromosome
Accession NZ_CP069132
Organism Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JQK35_RS08025 Protein ID WP_000323025.1
Coordinates 1639599..1639886 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JQK35_RS08030 Protein ID WP_000534858.1
Coordinates 1639886..1640125 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK35_RS07980 1634623..1634838 - 216 WP_000839590.1 phage lysis protein EssD -
JQK35_RS07985 1635592..1635807 - 216 WP_000066484.1 cold shock-like protein CspB -
JQK35_RS07990 1636108..1636320 + 213 WP_000087756.1 cold shock-like protein CspF -
JQK35_RS07995 1636375..1636464 + 90 WP_120795389.1 hypothetical protein -
JQK35_RS08000 1636742..1637494 - 753 WP_001047135.1 antitermination protein -
JQK35_RS08005 1637508..1638557 - 1050 WP_001393597.1 DUF968 domain-containing protein -
JQK35_RS08010 1638559..1638837 - 279 WP_012304870.1 hypothetical protein -
JQK35_RS08015 1638904..1639155 - 252 WP_000980994.1 hypothetical protein -
JQK35_RS08020 1639372..1639527 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JQK35_RS08025 1639599..1639886 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JQK35_RS08030 1639886..1640125 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JQK35_RS08035 1640150..1640455 + 306 WP_001326990.1 hypothetical protein -
JQK35_RS08040 1640658..1640990 + 333 WP_001301033.1 protein FlxA -
JQK35_RS08045 1641427..1641576 - 150 WP_011443592.1 hypothetical protein -
JQK35_RS08050 1641611..1641889 - 279 Protein_1573 hypothetical protein -
JQK35_RS08055 1641873..1642103 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
JQK35_RS08060 1642187..1642594 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
JQK35_RS08065 1642761..1642916 + 156 WP_000379575.1 DUF1391 family protein -
JQK35_RS08070 1643076..1643294 + 219 WP_001171942.1 hypothetical protein -
JQK35_RS08075 1643862..1644050 + 189 WP_000854559.1 cell division inhibition protein DicB -
JQK35_RS08080 1644047..1644238 + 192 WP_001083297.1 lysis protein YdfD -
JQK35_RS08085 1644331..1645098 + 768 Protein_1580 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625255..1658716 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T189919 WP_000323025.1 NZ_CP069132:c1639886-1639599 [Escherichia coli BW25113]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T189919 NZ_CP091026:2486418-2487740 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT189919 WP_000534858.1 NZ_CP069132:c1640125-1639886 [Escherichia coli BW25113]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT189919 NZ_CP091026:2486152-2486418 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References