189919

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639599..1640125 Replicon chromosome
Accession NZ_CP069132
Organism Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JQK35_RS08025 Protein ID WP_000323025.1
Coordinates 1639599..1639886 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JQK35_RS08030 Protein ID WP_000534858.1
Coordinates 1639886..1640125 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK35_RS07980 1634623..1634838 - 216 WP_000839590.1 phage lysis protein EssD -
JQK35_RS07985 1635592..1635807 - 216 WP_000066484.1 cold shock-like protein CspB -
JQK35_RS07990 1636108..1636320 + 213 WP_000087756.1 cold shock-like protein CspF -
JQK35_RS07995 1636375..1636464 + 90 WP_120795389.1 hypothetical protein -
JQK35_RS08000 1636742..1637494 - 753 WP_001047135.1 antitermination protein -
JQK35_RS08005 1637508..1638557 - 1050 WP_001393597.1 DUF968 domain-containing protein -
JQK35_RS08010 1638559..1638837 - 279 WP_012304870.1 hypothetical protein -
JQK35_RS08015 1638904..1639155 - 252 WP_000980994.1 hypothetical protein -
JQK35_RS08020 1639372..1639527 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JQK35_RS08025 1639599..1639886 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JQK35_RS08030 1639886..1640125 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JQK35_RS08035 1640150..1640455 + 306 WP_001326990.1 hypothetical protein -
JQK35_RS08040 1640658..1640990 + 333 WP_001301033.1 protein FlxA -
JQK35_RS08045 1641427..1641576 - 150 WP_011443592.1 hypothetical protein -
JQK35_RS08050 1641611..1641889 - 279 Protein_1573 hypothetical protein -
JQK35_RS08055 1641873..1642103 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
JQK35_RS08060 1642187..1642594 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
JQK35_RS08065 1642761..1642916 + 156 WP_000379575.1 DUF1391 family protein -
JQK35_RS08070 1643076..1643294 + 219 WP_001171942.1 hypothetical protein -
JQK35_RS08075 1643862..1644050 + 189 WP_000854559.1 cell division inhibition protein DicB -
JQK35_RS08080 1644047..1644238 + 192 WP_001083297.1 lysis protein YdfD -
JQK35_RS08085 1644331..1645098 + 768 Protein_1580 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625255..1658716 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T189919 WP_000323025.1 NZ_CP069132:c1639886-1639599 [Escherichia coli BW25113]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T189919 NZ_CP091026:2486418-2487740 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT189919 WP_000534858.1 NZ_CP069132:c1640125-1639886 [Escherichia coli BW25113]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT189919 NZ_CP091026:2486152-2486418 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References