Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2995793..2996319 Replicon chromosome
Accession NZ_CP012868
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag QQ24_RS14730 Protein ID WP_000323025.1
Coordinates 2996032..2996319 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag QQ24_RS14725 Protein ID WP_000534858.1
Coordinates 2995793..2996032 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQ24_RS24130 2990820..2991587 - 768 Protein_2777 exonuclease -
QQ24_RS14680 2991680..2991871 - 192 WP_001083297.1 lysis protein YdfD -
QQ24_RS14685 2991868..2992056 - 189 WP_000854559.1 cell division inhibition protein DicB -
QQ24_RS14695 2992624..2992842 - 219 WP_001171942.1 hypothetical protein -
QQ24_RS14700 2993002..2993157 - 156 WP_000379575.1 DUF1391 family protein -
QQ24_RS14705 2993324..2993731 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
QQ24_RS14710 2993815..2994045 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
QQ24_RS14715 2994029..2994307 + 279 Protein_2784 hypothetical protein -
QQ24_RS22950 2994342..2994491 + 150 WP_011443592.1 hypothetical protein -
QQ24_RS14720 2994928..2995260 - 333 WP_001301033.1 protein FlxA -
QQ24_RS24145 2995463..2995768 - 306 WP_001326990.1 hypothetical protein -
QQ24_RS14725 2995793..2996032 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
QQ24_RS14730 2996032..2996319 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
QQ24_RS14735 2996391..2996546 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
QQ24_RS14740 2996763..2997014 + 252 WP_000980994.1 hypothetical protein -
QQ24_RS14745 2997081..2997359 + 279 WP_012304870.1 hypothetical protein -
QQ24_RS14750 2997361..2998410 + 1050 WP_001393597.1 DUF968 domain-containing protein -
QQ24_RS14755 2998424..2999176 + 753 WP_001047135.1 antitermination protein -
QQ24_RS25645 2999454..2999543 - 90 WP_120795389.1 hypothetical protein -
QQ24_RS14760 2999598..2999810 - 213 WP_000087756.1 cold shock-like protein CspF -
QQ24_RS14765 3000111..3000326 + 216 WP_000066484.1 cold shock-like protein CspB -
QQ24_RS26170 3000690..3000860 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
QQ24_RS14770 3001080..3001295 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2977202..3010663 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T57308 WP_000323025.1 NZ_CP012868:2996032-2996319 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T57308 NZ_CP012868:2996032-2996319 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT57308 WP_000534858.1 NZ_CP012868:2995793-2996032 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT57308 NZ_CP012868:2995793-2996032 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References