Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2238799..2239325 Replicon chromosome
Accession NZ_CP043187
Organism Escherichia coli O16:H48 strain PG20180170

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FYA17_RS10845 Protein ID WP_000323025.1
Coordinates 2239038..2239325 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FYA17_RS10840 Protein ID WP_000534858.1
Coordinates 2238799..2239038 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA17_RS10780 2233826..2234593 - 768 Protein_2099 exonuclease -
FYA17_RS10785 2234686..2234877 - 192 WP_001083297.1 lysis protein YdfD -
FYA17_RS10790 2234874..2235062 - 189 WP_000854559.1 cell division inhibition protein DicB -
FYA17_RS10800 2235630..2235848 - 219 WP_001171942.1 hypothetical protein -
FYA17_RS10805 2236008..2236163 - 156 WP_000379575.1 DUF1391 family protein -
FYA17_RS10810 2236330..2236737 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FYA17_RS10815 2236821..2237051 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
FYA17_RS10820 2237035..2237313 + 279 Protein_2106 hypothetical protein -
FYA17_RS10825 2237348..2237497 + 150 WP_011443592.1 hypothetical protein -
FYA17_RS10830 2237934..2238266 - 333 WP_001301033.1 protein FlxA -
FYA17_RS10835 2238469..2238774 - 306 WP_001326990.1 hypothetical protein -
FYA17_RS10840 2238799..2239038 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FYA17_RS10845 2239038..2239325 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FYA17_RS10850 2239397..2239552 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FYA17_RS10855 2239769..2240020 + 252 WP_000980994.1 hypothetical protein -
FYA17_RS10860 2240087..2240365 + 279 WP_012304870.1 hypothetical protein -
FYA17_RS10865 2240367..2241416 + 1050 WP_001265198.1 DUF968 domain-containing protein -
FYA17_RS10870 2241430..2242182 + 753 WP_001047135.1 antitermination protein -
FYA17_RS10875 2242460..2242549 - 90 WP_120795389.1 hypothetical protein -
FYA17_RS10880 2242604..2242816 - 213 WP_000087756.1 cold shock-like protein CspF -
FYA17_RS10885 2243117..2243332 + 216 WP_000066484.1 cold shock-like protein CspB -
FYA17_RS23160 2243696..2243866 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
FYA17_RS10890 2244086..2244301 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2220208..2253669 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T137478 WP_000323025.1 NZ_CP043187:2239038-2239325 [Escherichia coli O16:H48]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T137478 NZ_CP043187:2239038-2239325 [Escherichia coli O16:H48]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT137478 WP_000534858.1 NZ_CP043187:2238799-2239038 [Escherichia coli O16:H48]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT137478 NZ_CP043187:2238799-2239038 [Escherichia coli O16:H48]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References