57370

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2995800..2996326 Replicon chromosome
Accession NZ_CP012870
Organism Escherichia coli strain K-12 substr. MG1655_TMP32XR2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag QR63_RS14735 Protein ID WP_000323025.1
Coordinates 2996039..2996326 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag QR63_RS14730 Protein ID WP_000534858.1
Coordinates 2995800..2996039 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QR63_RS24125 2990827..2991594 - 768 Protein_2777 exonuclease -
QR63_RS14685 2991687..2991878 - 192 WP_001083297.1 lysis protein YdfD -
QR63_RS14690 2991875..2992063 - 189 WP_000854559.1 cell division inhibition protein DicB -
QR63_RS14700 2992631..2992849 - 219 WP_001171942.1 hypothetical protein -
QR63_RS14705 2993009..2993164 - 156 WP_000379575.1 DUF1391 family protein -
QR63_RS14710 2993331..2993738 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
QR63_RS14715 2993822..2994052 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
QR63_RS14720 2994036..2994314 + 279 Protein_2784 hypothetical protein -
QR63_RS22955 2994349..2994498 + 150 WP_011443592.1 hypothetical protein -
QR63_RS14725 2994935..2995267 - 333 WP_001301033.1 protein FlxA -
QR63_RS24140 2995470..2995775 - 306 WP_001326990.1 hypothetical protein -
QR63_RS14730 2995800..2996039 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
QR63_RS14735 2996039..2996326 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
QR63_RS14740 2996398..2996553 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
QR63_RS14745 2996770..2997021 + 252 WP_000980994.1 hypothetical protein -
QR63_RS14750 2997088..2997366 + 279 WP_012304870.1 hypothetical protein -
QR63_RS14755 2997368..2998417 + 1050 WP_001393597.1 DUF968 domain-containing protein -
QR63_RS14760 2998431..2999183 + 753 WP_001047135.1 antitermination protein -
QR63_RS25645 2999461..2999550 - 90 WP_120795389.1 hypothetical protein -
QR63_RS14765 2999605..2999817 - 213 WP_000087756.1 cold shock-like protein CspF -
QR63_RS14770 3000118..3000333 + 216 WP_000066484.1 cold shock-like protein CspB -
QR63_RS26170 3000697..3000867 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
QR63_RS14775 3001087..3001302 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2977209..3010670 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T57370 WP_000323025.1 NZ_CP012870:2996039-2996326 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T57370 NZ_CP012870:2996039-2996326 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT57370 WP_000534858.1 NZ_CP012870:2995800-2996039 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT57370 NZ_CP012870:2995800-2996039 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References