52469

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2385826..2386352 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH02_RS11860 Protein ID WP_000323025.1
Coordinates 2385826..2386113 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH02_RS11865 Protein ID WP_000534858.1
Coordinates 2386113..2386352 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS11820 2380850..2381065 - 216 WP_000839590.1 phage lysis protein EssD -
SH02_RS11825 2381819..2382034 - 216 WP_000066484.1 cold shock-like protein CspB -
SH02_RS11830 2382335..2382547 + 213 WP_000087756.1 cold shock-like protein CspF -
SH02_RS25680 2382602..2382691 + 90 WP_120795389.1 hypothetical protein -
SH02_RS11835 2382969..2383721 - 753 WP_001047135.1 antitermination protein -
SH02_RS11840 2383735..2384784 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH02_RS11845 2384786..2385064 - 279 WP_012304870.1 hypothetical protein -
SH02_RS11850 2385131..2385382 - 252 WP_000980994.1 hypothetical protein -
SH02_RS11855 2385599..2385754 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH02_RS11860 2385826..2386113 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH02_RS11865 2386113..2386352 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH02_RS24110 2386377..2386682 + 306 WP_001326990.1 hypothetical protein -
SH02_RS11870 2386885..2387217 + 333 WP_001301033.1 protein FlxA -
SH02_RS23030 2387654..2387803 - 150 WP_011443592.1 hypothetical protein -
SH02_RS11875 2387838..2388116 - 279 Protein_2241 hypothetical protein -
SH02_RS11880 2388100..2388330 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH02_RS11885 2388414..2388821 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH02_RS11890 2388988..2389143 + 156 WP_000379575.1 DUF1391 family protein -
SH02_RS11895 2389303..2389521 + 219 WP_001171942.1 hypothetical protein -
SH02_RS11905 2390089..2390277 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH02_RS11910 2390274..2390465 + 192 WP_038432577.1 lysis protein YdfD -
SH02_RS24125 2390558..2391325 + 768 Protein_2248 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2371482..2397224 25742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52469 WP_000323025.1 NZ_CP010439:c2386113-2385826 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52469 NZ_CP010439:c2386113-2385826 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52469 WP_000534858.1 NZ_CP010439:c2386352-2386113 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52469 NZ_CP010439:c2386352-2386113 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References