Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2385826..2386352 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH02_RS11860 Protein ID WP_000323025.1
Coordinates 2385826..2386113 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH02_RS11865 Protein ID WP_000534858.1
Coordinates 2386113..2386352 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS11820 2380850..2381065 - 216 WP_000839590.1 phage lysis protein EssD -
SH02_RS11825 2381819..2382034 - 216 WP_000066484.1 cold shock-like protein CspB -
SH02_RS11830 2382335..2382547 + 213 WP_000087756.1 cold shock-like protein CspF -
SH02_RS25680 2382602..2382691 + 90 WP_120795389.1 hypothetical protein -
SH02_RS11835 2382969..2383721 - 753 WP_001047135.1 antitermination protein -
SH02_RS11840 2383735..2384784 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH02_RS11845 2384786..2385064 - 279 WP_012304870.1 hypothetical protein -
SH02_RS11850 2385131..2385382 - 252 WP_000980994.1 hypothetical protein -
SH02_RS11855 2385599..2385754 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH02_RS11860 2385826..2386113 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH02_RS11865 2386113..2386352 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH02_RS24110 2386377..2386682 + 306 WP_001326990.1 hypothetical protein -
SH02_RS11870 2386885..2387217 + 333 WP_001301033.1 protein FlxA -
SH02_RS23030 2387654..2387803 - 150 WP_011443592.1 hypothetical protein -
SH02_RS11875 2387838..2388116 - 279 Protein_2241 hypothetical protein -
SH02_RS11880 2388100..2388330 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH02_RS11885 2388414..2388821 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH02_RS11890 2388988..2389143 + 156 WP_000379575.1 DUF1391 family protein -
SH02_RS11895 2389303..2389521 + 219 WP_001171942.1 hypothetical protein -
SH02_RS11905 2390089..2390277 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH02_RS11910 2390274..2390465 + 192 WP_038432577.1 lysis protein YdfD -
SH02_RS24125 2390558..2391325 + 768 Protein_2248 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2371482..2397224 25742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52469 WP_000323025.1 NZ_CP010439:c2386113-2385826 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52469 NZ_CP010439:c2386113-2385826 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52469 WP_000534858.1 NZ_CP010439:c2386352-2386113 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52469 NZ_CP010439:c2386352-2386113 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References