136220

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2344841..2345367 Replicon chromosome
Accession NZ_CP042846
Organism Escherichia coli strain JME64

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FVE20_RS11425 Protein ID WP_000323025.1
Coordinates 2344841..2345128 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FVE20_RS11430 Protein ID WP_000534858.1
Coordinates 2345128..2345367 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE20_RS11380 2339865..2340080 - 216 WP_000839590.1 phage lysis protein EssD -
FVE20_RS11385 2340834..2341049 - 216 WP_000066484.1 cold shock-like protein CspB -
FVE20_RS11390 2341350..2341562 + 213 WP_000087756.1 cold shock-like protein CspF -
FVE20_RS11395 2341617..2341706 + 90 WP_120795389.1 hypothetical protein -
FVE20_RS11400 2341984..2342736 - 753 WP_001047135.1 antitermination protein -
FVE20_RS11405 2342750..2343799 - 1050 WP_001393597.1 DUF968 domain-containing protein -
FVE20_RS11410 2343801..2344079 - 279 WP_012304870.1 hypothetical protein -
FVE20_RS11415 2344146..2344397 - 252 WP_000980994.1 hypothetical protein -
FVE20_RS11420 2344614..2344769 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FVE20_RS11425 2344841..2345128 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FVE20_RS11430 2345128..2345367 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FVE20_RS11435 2345392..2345697 + 306 WP_001326990.1 hypothetical protein -
FVE20_RS11440 2345900..2346232 + 333 WP_001301033.1 protein FlxA -
FVE20_RS11445 2346669..2346818 - 150 WP_011443592.1 hypothetical protein -
FVE20_RS11450 2346853..2347131 - 279 Protein_2199 hypothetical protein -
FVE20_RS11455 2347115..2347345 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FVE20_RS11460 2347429..2347836 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FVE20_RS11465 2348003..2348158 + 156 WP_000379575.1 DUF1391 family protein -
FVE20_RS11470 2348318..2348536 + 219 WP_001171942.1 hypothetical protein -
FVE20_RS11480 2349104..2349292 + 189 WP_000854559.1 cell division inhibition protein DicB -
FVE20_RS11485 2349289..2349480 + 192 WP_001083297.1 lysis protein YdfD -
FVE20_RS11490 2349573..2350340 + 768 Protein_2206 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2330497..2363958 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T136220 WP_000323025.1 NZ_CP042846:c2345128-2344841 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T136220 NZ_CP042846:c2345128-2344841 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT136220 WP_000534858.1 NZ_CP042846:c2345367-2345128 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT136220 NZ_CP042846:c2345367-2345128 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References