Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2344841..2345367 Replicon chromosome
Accession NZ_CP042846
Organism Escherichia coli strain JME64

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FVE20_RS11425 Protein ID WP_000323025.1
Coordinates 2344841..2345128 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FVE20_RS11430 Protein ID WP_000534858.1
Coordinates 2345128..2345367 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE20_RS11380 2339865..2340080 - 216 WP_000839590.1 phage lysis protein EssD -
FVE20_RS11385 2340834..2341049 - 216 WP_000066484.1 cold shock-like protein CspB -
FVE20_RS11390 2341350..2341562 + 213 WP_000087756.1 cold shock-like protein CspF -
FVE20_RS11395 2341617..2341706 + 90 WP_120795389.1 hypothetical protein -
FVE20_RS11400 2341984..2342736 - 753 WP_001047135.1 antitermination protein -
FVE20_RS11405 2342750..2343799 - 1050 WP_001393597.1 DUF968 domain-containing protein -
FVE20_RS11410 2343801..2344079 - 279 WP_012304870.1 hypothetical protein -
FVE20_RS11415 2344146..2344397 - 252 WP_000980994.1 hypothetical protein -
FVE20_RS11420 2344614..2344769 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FVE20_RS11425 2344841..2345128 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FVE20_RS11430 2345128..2345367 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FVE20_RS11435 2345392..2345697 + 306 WP_001326990.1 hypothetical protein -
FVE20_RS11440 2345900..2346232 + 333 WP_001301033.1 protein FlxA -
FVE20_RS11445 2346669..2346818 - 150 WP_011443592.1 hypothetical protein -
FVE20_RS11450 2346853..2347131 - 279 Protein_2199 hypothetical protein -
FVE20_RS11455 2347115..2347345 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FVE20_RS11460 2347429..2347836 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FVE20_RS11465 2348003..2348158 + 156 WP_000379575.1 DUF1391 family protein -
FVE20_RS11470 2348318..2348536 + 219 WP_001171942.1 hypothetical protein -
FVE20_RS11480 2349104..2349292 + 189 WP_000854559.1 cell division inhibition protein DicB -
FVE20_RS11485 2349289..2349480 + 192 WP_001083297.1 lysis protein YdfD -
FVE20_RS11490 2349573..2350340 + 768 Protein_2206 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2330497..2363958 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T136220 WP_000323025.1 NZ_CP042846:c2345128-2344841 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T136220 NZ_CP042846:c2345128-2344841 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT136220 WP_000534858.1 NZ_CP042846:c2345367-2345128 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT136220 NZ_CP042846:c2345367-2345128 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References