27170

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2232606..2233132 Replicon chromosome
Accession NC_017625
Organism Escherichia coli DH1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECDH1_RS11025 Protein ID WP_000323025.1
Coordinates 2232845..2233132 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECDH1_RS11020 Protein ID WP_000534858.1
Coordinates 2232606..2232845 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1_RS23845 2227633..2228400 - 768 Protein_2083 exonuclease -
ECDH1_RS10975 2228493..2228684 - 192 WP_001083297.1 lysis protein YdfD -
ECDH1_RS10980 2228681..2228869 - 189 WP_000854559.1 cell division inhibition protein DicB -
ECDH1_RS10990 2229437..2229655 - 219 WP_001171942.1 hypothetical protein -
ECDH1_RS10995 2229815..2229970 - 156 WP_000379575.1 DUF1391 family protein -
ECDH1_RS11000 2230137..2230544 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ECDH1_RS11005 2230628..2230858 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
ECDH1_RS11010 2230842..2231120 + 279 Protein_2090 hypothetical protein -
ECDH1_RS22990 2231155..2231304 + 150 WP_011443592.1 hypothetical protein -
ECDH1_RS11015 2231741..2232073 - 333 WP_001301033.1 protein FlxA -
ECDH1_RS23860 2232276..2232581 - 306 WP_001326990.1 hypothetical protein -
ECDH1_RS11020 2232606..2232845 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECDH1_RS11025 2232845..2233132 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECDH1_RS11030 2233204..2233359 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ECDH1_RS11035 2233576..2233827 + 252 WP_000980994.1 hypothetical protein -
ECDH1_RS11040 2233894..2234172 + 279 WP_012304870.1 hypothetical protein -
ECDH1_RS11045 2234174..2235223 + 1050 WP_001265198.1 DUF968 domain-containing protein -
ECDH1_RS11050 2235237..2235989 + 753 WP_001047135.1 antitermination protein -
ECDH1_RS25630 2236267..2236356 - 90 WP_120795389.1 hypothetical protein -
ECDH1_RS11055 2236411..2236623 - 213 WP_000087756.1 cold shock-like protein CspF -
ECDH1_RS11060 2236924..2237139 + 216 WP_000066484.1 cold shock-like protein CspB -
ECDH1_RS11065 2237893..2238108 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2221734..2249263 27529


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T27170 WP_000323025.1 NC_017625:2232845-2233132 [Escherichia coli DH1]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T27170 NC_017625:2232845-2233132 [Escherichia coli DH1]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT27170 WP_000534858.1 NC_017625:2232606-2232845 [Escherichia coli DH1]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT27170 NC_017625:2232606-2232845 [Escherichia coli DH1]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References