Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2232606..2233132 Replicon chromosome
Accession NC_017625
Organism Escherichia coli DH1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECDH1_RS11025 Protein ID WP_000323025.1
Coordinates 2232845..2233132 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECDH1_RS11020 Protein ID WP_000534858.1
Coordinates 2232606..2232845 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1_RS23845 2227633..2228400 - 768 Protein_2083 exonuclease -
ECDH1_RS10975 2228493..2228684 - 192 WP_001083297.1 lysis protein YdfD -
ECDH1_RS10980 2228681..2228869 - 189 WP_000854559.1 cell division inhibition protein DicB -
ECDH1_RS10990 2229437..2229655 - 219 WP_001171942.1 hypothetical protein -
ECDH1_RS10995 2229815..2229970 - 156 WP_000379575.1 DUF1391 family protein -
ECDH1_RS11000 2230137..2230544 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ECDH1_RS11005 2230628..2230858 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
ECDH1_RS11010 2230842..2231120 + 279 Protein_2090 hypothetical protein -
ECDH1_RS22990 2231155..2231304 + 150 WP_011443592.1 hypothetical protein -
ECDH1_RS11015 2231741..2232073 - 333 WP_001301033.1 protein FlxA -
ECDH1_RS23860 2232276..2232581 - 306 WP_001326990.1 hypothetical protein -
ECDH1_RS11020 2232606..2232845 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECDH1_RS11025 2232845..2233132 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECDH1_RS11030 2233204..2233359 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ECDH1_RS11035 2233576..2233827 + 252 WP_000980994.1 hypothetical protein -
ECDH1_RS11040 2233894..2234172 + 279 WP_012304870.1 hypothetical protein -
ECDH1_RS11045 2234174..2235223 + 1050 WP_001265198.1 DUF968 domain-containing protein -
ECDH1_RS11050 2235237..2235989 + 753 WP_001047135.1 antitermination protein -
ECDH1_RS25630 2236267..2236356 - 90 WP_120795389.1 hypothetical protein -
ECDH1_RS11055 2236411..2236623 - 213 WP_000087756.1 cold shock-like protein CspF -
ECDH1_RS11060 2236924..2237139 + 216 WP_000066484.1 cold shock-like protein CspB -
ECDH1_RS11065 2237893..2238108 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2221734..2249263 27529


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T27170 WP_000323025.1 NC_017625:2232845-2233132 [Escherichia coli DH1]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T27170 NC_017625:2232845-2233132 [Escherichia coli DH1]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT27170 WP_000534858.1 NC_017625:2232606-2232845 [Escherichia coli DH1]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT27170 NC_017625:2232606-2232845 [Escherichia coli DH1]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References