Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1650165..1650691 Replicon chromosome
Accession NZ_CP025268
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CXP41_RS08700 Protein ID WP_000323025.1
Coordinates 1650165..1650452 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CXP41_RS08705 Protein ID WP_000534858.1
Coordinates 1650452..1650691 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXP41_RS08660 1645189..1645404 - 216 WP_000839590.1 phage lysis protein EssD -
CXP41_RS24470 1645624..1645794 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
CXP41_RS08665 1646158..1646373 - 216 WP_000066484.1 cold shock-like protein CspB -
CXP41_RS08670 1646674..1646886 + 213 WP_000087756.1 cold shock-like protein CspF -
CXP41_RS24205 1646941..1647030 + 90 WP_120795389.1 hypothetical protein -
CXP41_RS08675 1647308..1648060 - 753 WP_001047135.1 antitermination protein -
CXP41_RS08680 1648074..1649123 - 1050 WP_001393597.1 DUF968 domain-containing protein -
CXP41_RS08685 1649125..1649403 - 279 WP_012304870.1 hypothetical protein -
CXP41_RS08690 1649470..1649721 - 252 WP_000980994.1 hypothetical protein -
CXP41_RS08695 1649938..1650093 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CXP41_RS08700 1650165..1650452 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CXP41_RS08705 1650452..1650691 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CXP41_RS08710 1650716..1651021 + 306 WP_001326990.1 hypothetical protein -
CXP41_RS08715 1651224..1651556 + 333 WP_001301033.1 protein FlxA -
CXP41_RS24210 1651993..1652142 - 150 WP_011443592.1 hypothetical protein -
CXP41_RS08725 1652177..1652455 - 279 Protein_1601 hypothetical protein -
CXP41_RS08730 1652439..1652669 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
CXP41_RS08735 1652753..1653160 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
CXP41_RS08740 1653327..1653482 + 156 WP_000379575.1 DUF1391 family protein -
CXP41_RS08750 1653642..1653860 + 219 WP_001171942.1 hypothetical protein -
CXP41_RS08760 1654428..1654616 + 189 WP_000854559.1 cell division inhibition protein DicB -
CXP41_RS08765 1654613..1654804 + 192 WP_001083297.1 lysis protein YdfD -
CXP41_RS08770 1654897..1655664 + 768 Protein_1608 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1635821..1669281 33460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T89996 WP_000323025.1 NZ_CP025268:c1650452-1650165 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T89996 NZ_CP025268:c1650452-1650165 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT89996 WP_000534858.1 NZ_CP025268:c1650691-1650452 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT89996 NZ_CP025268:c1650691-1650452 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References