89996

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1650165..1650691 Replicon chromosome
Accession NZ_CP025268
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CXP41_RS08700 Protein ID WP_000323025.1
Coordinates 1650165..1650452 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CXP41_RS08705 Protein ID WP_000534858.1
Coordinates 1650452..1650691 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXP41_RS08660 1645189..1645404 - 216 WP_000839590.1 phage lysis protein EssD -
CXP41_RS24470 1645624..1645794 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
CXP41_RS08665 1646158..1646373 - 216 WP_000066484.1 cold shock-like protein CspB -
CXP41_RS08670 1646674..1646886 + 213 WP_000087756.1 cold shock-like protein CspF -
CXP41_RS24205 1646941..1647030 + 90 WP_120795389.1 hypothetical protein -
CXP41_RS08675 1647308..1648060 - 753 WP_001047135.1 antitermination protein -
CXP41_RS08680 1648074..1649123 - 1050 WP_001393597.1 DUF968 domain-containing protein -
CXP41_RS08685 1649125..1649403 - 279 WP_012304870.1 hypothetical protein -
CXP41_RS08690 1649470..1649721 - 252 WP_000980994.1 hypothetical protein -
CXP41_RS08695 1649938..1650093 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CXP41_RS08700 1650165..1650452 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CXP41_RS08705 1650452..1650691 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CXP41_RS08710 1650716..1651021 + 306 WP_001326990.1 hypothetical protein -
CXP41_RS08715 1651224..1651556 + 333 WP_001301033.1 protein FlxA -
CXP41_RS24210 1651993..1652142 - 150 WP_011443592.1 hypothetical protein -
CXP41_RS08725 1652177..1652455 - 279 Protein_1601 hypothetical protein -
CXP41_RS08730 1652439..1652669 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
CXP41_RS08735 1652753..1653160 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
CXP41_RS08740 1653327..1653482 + 156 WP_000379575.1 DUF1391 family protein -
CXP41_RS08750 1653642..1653860 + 219 WP_001171942.1 hypothetical protein -
CXP41_RS08760 1654428..1654616 + 189 WP_000854559.1 cell division inhibition protein DicB -
CXP41_RS08765 1654613..1654804 + 192 WP_001083297.1 lysis protein YdfD -
CXP41_RS08770 1654897..1655664 + 768 Protein_1608 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1635821..1669281 33460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T89996 WP_000323025.1 NZ_CP025268:c1650452-1650165 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T89996 NZ_CP025268:c1650452-1650165 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT89996 WP_000534858.1 NZ_CP025268:c1650691-1650452 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT89996 NZ_CP025268:c1650691-1650452 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References