Detailed information of TA system TA112075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2236725..2237251
• Replicon: chromosome
• Accession: NZ_CP032667
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: D8B36_RS11090
• Protein ID: WP_000323025.1
• Coordinates: 2236964..2237251 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: D8B36_RS11085
• Protein ID: WP_000534858.1
• Coordinates: 2236725..2236964 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8B36_RS11025	2231752..2232519	-	768	Protein_2098	exonuclease	-
D8B36_RS11030	2232612..2232803	-	192	WP_001083297.1	lysis protein YdfD	-
D8B36_RS11035	2232800..2232988	-	189	WP_000854559.1	cell division inhibition protein DicB	-
D8B36_RS11045	2233556..2233774	-	219	WP_001171942.1	hypothetical protein	-
D8B36_RS11055	2233934..2234089	-	156	WP_000379575.1	DUF1391 family protein	-
D8B36_RS11060	2234256..2234663	-	408	WP_000448564.1	DNA-binding transcriptional dual regulator DicA	-
D8B36_RS11065	2234747..2234977	+	231	WP_000920568.1	dicB transcriptional regulator DicC	-
D8B36_RS11070	2234961..2235239	+	279	Protein_2105	hypothetical protein	-
D8B36_RS22995	2235274..2235423	+	150	WP_011443592.1	hypothetical protein	-
D8B36_RS11075	2235860..2236192	-	333	WP_001301033.1	protein FlxA	-
D8B36_RS11080	2236395..2236700	-	306	WP_001326990.1	hypothetical protein	-
D8B36_RS11085	2236725..2236964	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
D8B36_RS11090	2236964..2237251	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
D8B36_RS11095	2237323..2237478	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
D8B36_RS11100	2237695..2237946	+	252	WP_000980994.1	hypothetical protein	-
D8B36_RS11105	2238013..2238291	+	279	WP_012304870.1	hypothetical protein	-
D8B36_RS11110	2238293..2239342	+	1050	WP_001393597.1	DUF968 domain-containing protein	-
D8B36_RS11115	2239356..2240108	+	753	WP_001047135.1	antitermination protein	-
D8B36_RS23000	2240386..2240475	-	90	WP_120795389.1	hypothetical protein	-
D8B36_RS11120	2240530..2240742	-	213	WP_000087756.1	cold shock-like protein CspF	-
D8B36_RS11125	2241043..2241258	+	216	WP_000066484.1	cold shock-like protein CspB	-
D8B36_RS23330	2241622..2241792	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
D8B36_RS11130	2242012..2242227	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Prophage TA020067 1519531..1552992 0.004 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Prophage TA001041 1631002..1664463 0.004 View NC_010473 Escherichia coli str. K-12 substr. DH10B Prophage TA022686 1718651..1753448 0.005 View NC_012759 Escherichia coli BW2952 Prophage TA023854 1521085..1554546 0.004 View NZ_CP009273 Escherichia coli BW25113 Prophage TA048683 1625259..1658720 0.004 View NZ_CP020543 Escherichia coli C Prophage TA075071 2249542..2301270 0.009 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Prophage TA285017 1519531..1552992 0.004	inside	Prophage	-	-	2218134..2258492	40358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T112075 WP_000323025.1 NZ_CP032667:2236964-2237251 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T112075 NZ_CP032667:2236964-2237251 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT112075 WP_000534858.1 NZ_CP032667:2236725-2236964 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT112075 NZ_CP032667:2236725-2236964 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8