Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2236725..2237251 Replicon chromosome
Accession NZ_CP032667
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D8B36_RS11090 Protein ID WP_000323025.1
Coordinates 2236964..2237251 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D8B36_RS11085 Protein ID WP_000534858.1
Coordinates 2236725..2236964 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8B36_RS11025 2231752..2232519 - 768 Protein_2098 exonuclease -
D8B36_RS11030 2232612..2232803 - 192 WP_001083297.1 lysis protein YdfD -
D8B36_RS11035 2232800..2232988 - 189 WP_000854559.1 cell division inhibition protein DicB -
D8B36_RS11045 2233556..2233774 - 219 WP_001171942.1 hypothetical protein -
D8B36_RS11055 2233934..2234089 - 156 WP_000379575.1 DUF1391 family protein -
D8B36_RS11060 2234256..2234663 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
D8B36_RS11065 2234747..2234977 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
D8B36_RS11070 2234961..2235239 + 279 Protein_2105 hypothetical protein -
D8B36_RS22995 2235274..2235423 + 150 WP_011443592.1 hypothetical protein -
D8B36_RS11075 2235860..2236192 - 333 WP_001301033.1 protein FlxA -
D8B36_RS11080 2236395..2236700 - 306 WP_001326990.1 hypothetical protein -
D8B36_RS11085 2236725..2236964 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D8B36_RS11090 2236964..2237251 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D8B36_RS11095 2237323..2237478 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
D8B36_RS11100 2237695..2237946 + 252 WP_000980994.1 hypothetical protein -
D8B36_RS11105 2238013..2238291 + 279 WP_012304870.1 hypothetical protein -
D8B36_RS11110 2238293..2239342 + 1050 WP_001393597.1 DUF968 domain-containing protein -
D8B36_RS11115 2239356..2240108 + 753 WP_001047135.1 antitermination protein -
D8B36_RS23000 2240386..2240475 - 90 WP_120795389.1 hypothetical protein -
D8B36_RS11120 2240530..2240742 - 213 WP_000087756.1 cold shock-like protein CspF -
D8B36_RS11125 2241043..2241258 + 216 WP_000066484.1 cold shock-like protein CspB -
D8B36_RS23330 2241622..2241792 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
D8B36_RS11130 2242012..2242227 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2218134..2258492 40358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T112075 WP_000323025.1 NZ_CP032667:2236964-2237251 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T112075 NZ_CP032667:2236964-2237251 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT112075 WP_000534858.1 NZ_CP032667:2236725-2236964 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT112075 NZ_CP032667:2236725-2236964 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References