Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2354835..2355361 Replicon chromosome
Accession NZ_CP042843
Organism Escherichia coli strain JME67

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FVE23_RS11480 Protein ID WP_000323025.1
Coordinates 2354835..2355122 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FVE23_RS11485 Protein ID WP_000534858.1
Coordinates 2355122..2355361 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE23_RS11435 2349859..2350074 - 216 WP_000839590.1 phage lysis protein EssD -
FVE23_RS11440 2350828..2351043 - 216 WP_000066484.1 cold shock-like protein CspB -
FVE23_RS11445 2351344..2351556 + 213 WP_000087756.1 cold shock-like protein CspF -
FVE23_RS11450 2351611..2351700 + 90 WP_120795389.1 hypothetical protein -
FVE23_RS11455 2351978..2352730 - 753 WP_001047135.1 antitermination protein -
FVE23_RS11460 2352744..2353793 - 1050 WP_001393597.1 DUF968 domain-containing protein -
FVE23_RS11465 2353795..2354073 - 279 WP_012304870.1 hypothetical protein -
FVE23_RS11470 2354140..2354391 - 252 WP_000980994.1 hypothetical protein -
FVE23_RS11475 2354608..2354763 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FVE23_RS11480 2354835..2355122 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FVE23_RS11485 2355122..2355361 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FVE23_RS11490 2355386..2355691 + 306 WP_001326990.1 hypothetical protein -
FVE23_RS11495 2355894..2356226 + 333 WP_001301033.1 protein FlxA -
FVE23_RS11500 2356663..2356812 - 150 WP_011443592.1 hypothetical protein -
FVE23_RS11505 2356847..2357125 - 279 Protein_2210 hypothetical protein -
FVE23_RS11510 2357109..2357339 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FVE23_RS11515 2357423..2357830 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FVE23_RS11520 2357997..2358152 + 156 WP_000379575.1 DUF1391 family protein -
FVE23_RS11525 2358312..2358530 + 219 WP_001171942.1 hypothetical protein -
FVE23_RS11535 2359098..2359286 + 189 WP_000854559.1 cell division inhibition protein DicB -
FVE23_RS11540 2359283..2359474 + 192 WP_001083297.1 lysis protein YdfD -
FVE23_RS11545 2359567..2360334 + 768 Protein_2217 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2340491..2373952 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T136091 WP_000323025.1 NZ_CP042843:c2355122-2354835 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T136091 NZ_CP042843:c2355122-2354835 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT136091 WP_000534858.1 NZ_CP042843:c2355361-2355122 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT136091 NZ_CP042843:c2355361-2355122 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References