60414

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4484270..4484796 Replicon chromosome
Accession NZ_CP014225
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AW869_RS22190 Protein ID WP_000323025.1
Coordinates 4484270..4484557 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AW869_RS22195 Protein ID WP_000534858.1
Coordinates 4484557..4484796 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW869_RS22150 4479294..4479509 - 216 WP_000839590.1 phage lysis protein EssD -
AW869_RS26230 4479729..4479899 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
AW869_RS22155 4480263..4480478 - 216 WP_000066484.1 cold shock-like protein CspB -
AW869_RS22160 4480779..4480991 + 213 WP_000087756.1 cold shock-like protein CspF -
AW869_RS25890 4481046..4481135 + 90 WP_120795389.1 hypothetical protein -
AW869_RS22165 4481413..4482165 - 753 WP_001047135.1 antitermination protein -
AW869_RS22170 4482179..4483228 - 1050 WP_001393597.1 DUF968 domain-containing protein -
AW869_RS22175 4483230..4483508 - 279 WP_012304870.1 hypothetical protein -
AW869_RS22180 4483575..4483826 - 252 WP_000980994.1 hypothetical protein -
AW869_RS22185 4484043..4484198 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
AW869_RS22190 4484270..4484557 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AW869_RS22195 4484557..4484796 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AW869_RS24835 4484821..4485126 + 306 WP_001326990.1 hypothetical protein -
AW869_RS22200 4485329..4485661 + 333 WP_001301033.1 protein FlxA -
AW869_RS22205 4486098..4486247 - 150 WP_011443592.1 hypothetical protein -
AW869_RS22210 4486282..4486560 - 279 Protein_4219 hypothetical protein -
AW869_RS22215 4486544..4486774 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
AW869_RS22220 4486858..4487265 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
AW869_RS22225 4487432..4487587 + 156 WP_000379575.1 DUF1391 family protein -
AW869_RS22230 4487747..4487965 + 219 WP_001171942.1 hypothetical protein -
AW869_RS22240 4488533..4488721 + 189 WP_000854559.1 cell division inhibition protein DicB -
AW869_RS22245 4488718..4488909 + 192 WP_001083297.1 lysis protein YdfD -
AW869_RS24850 4489002..4489769 + 768 Protein_4226 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4469926..4503387 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T60414 WP_000323025.1 NZ_CP014225:c4484557-4484270 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T60414 NZ_CP014225:c4484557-4484270 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT60414 WP_000534858.1 NZ_CP014225:c4484796-4484557 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT60414 NZ_CP014225:c4484796-4484557 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References