Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4484270..4484796 Replicon chromosome
Accession NZ_CP014225
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AW869_RS22190 Protein ID WP_000323025.1
Coordinates 4484270..4484557 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AW869_RS22195 Protein ID WP_000534858.1
Coordinates 4484557..4484796 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW869_RS22150 4479294..4479509 - 216 WP_000839590.1 phage lysis protein EssD -
AW869_RS26230 4479729..4479899 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
AW869_RS22155 4480263..4480478 - 216 WP_000066484.1 cold shock-like protein CspB -
AW869_RS22160 4480779..4480991 + 213 WP_000087756.1 cold shock-like protein CspF -
AW869_RS25890 4481046..4481135 + 90 WP_120795389.1 hypothetical protein -
AW869_RS22165 4481413..4482165 - 753 WP_001047135.1 antitermination protein -
AW869_RS22170 4482179..4483228 - 1050 WP_001393597.1 DUF968 domain-containing protein -
AW869_RS22175 4483230..4483508 - 279 WP_012304870.1 hypothetical protein -
AW869_RS22180 4483575..4483826 - 252 WP_000980994.1 hypothetical protein -
AW869_RS22185 4484043..4484198 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
AW869_RS22190 4484270..4484557 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AW869_RS22195 4484557..4484796 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AW869_RS24835 4484821..4485126 + 306 WP_001326990.1 hypothetical protein -
AW869_RS22200 4485329..4485661 + 333 WP_001301033.1 protein FlxA -
AW869_RS22205 4486098..4486247 - 150 WP_011443592.1 hypothetical protein -
AW869_RS22210 4486282..4486560 - 279 Protein_4219 hypothetical protein -
AW869_RS22215 4486544..4486774 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
AW869_RS22220 4486858..4487265 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
AW869_RS22225 4487432..4487587 + 156 WP_000379575.1 DUF1391 family protein -
AW869_RS22230 4487747..4487965 + 219 WP_001171942.1 hypothetical protein -
AW869_RS22240 4488533..4488721 + 189 WP_000854559.1 cell division inhibition protein DicB -
AW869_RS22245 4488718..4488909 + 192 WP_001083297.1 lysis protein YdfD -
AW869_RS24850 4489002..4489769 + 768 Protein_4226 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4469926..4503387 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T60414 WP_000323025.1 NZ_CP014225:c4484557-4484270 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T60414 NZ_CP014225:c4484557-4484270 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT60414 WP_000534858.1 NZ_CP014225:c4484796-4484557 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT60414 NZ_CP014225:c4484796-4484557 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References