Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639602..1640128 Replicon chromosome
Accession NZ_CP062245
Organism Escherichia coli strain AML003_ev01

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IHN50_RS08030 Protein ID WP_000323025.1
Coordinates 1639602..1639889 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IHN50_RS08035 Protein ID WP_000534858.1
Coordinates 1639889..1640128 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN50_RS07985 1634626..1634841 - 216 WP_000839590.1 phage lysis protein EssD -
IHN50_RS07990 1635595..1635810 - 216 WP_000066484.1 cold shock-like protein CspB -
IHN50_RS07995 1636111..1636323 + 213 WP_000087756.1 cold shock-like protein CspF -
IHN50_RS08000 1636378..1636467 + 90 WP_120795389.1 hypothetical protein -
IHN50_RS08005 1636745..1637497 - 753 WP_001047135.1 antitermination protein -
IHN50_RS08010 1637511..1638560 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IHN50_RS08015 1638562..1638840 - 279 WP_012304870.1 hypothetical protein -
IHN50_RS08020 1638907..1639158 - 252 WP_000980994.1 hypothetical protein -
IHN50_RS08025 1639375..1639530 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IHN50_RS08030 1639602..1639889 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IHN50_RS08035 1639889..1640128 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IHN50_RS08040 1640153..1640458 + 306 WP_001326990.1 hypothetical protein -
IHN50_RS08045 1640661..1640993 + 333 WP_001301033.1 protein FlxA -
IHN50_RS08050 1641430..1641579 - 150 WP_011443592.1 hypothetical protein -
IHN50_RS08055 1641614..1641892 - 279 Protein_1574 hypothetical protein -
IHN50_RS08060 1641876..1642106 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IHN50_RS08065 1642190..1642597 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IHN50_RS08070 1642764..1642919 + 156 WP_000379575.1 DUF1391 family protein -
IHN50_RS08075 1643079..1643297 + 219 WP_001171942.1 hypothetical protein -
IHN50_RS08080 1643865..1644053 + 189 WP_000854559.1 cell division inhibition protein DicB -
IHN50_RS08085 1644050..1644241 + 192 WP_001083297.1 lysis protein YdfD -
IHN50_RS08090 1644334..1645101 + 768 Protein_1581 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625258..1658719 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T173904 WP_000323025.1 NZ_CP062245:c1639889-1639602 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T173904 NZ_CP080372:72061-72441 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT173904 WP_000534858.1 NZ_CP062245:c1640128-1639889 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT173904 NZ_CP080372:71840-72061 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References