173904

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639602..1640128 Replicon chromosome
Accession NZ_CP062245
Organism Escherichia coli strain AML003_ev01

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IHN50_RS08030 Protein ID WP_000323025.1
Coordinates 1639602..1639889 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IHN50_RS08035 Protein ID WP_000534858.1
Coordinates 1639889..1640128 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN50_RS07985 1634626..1634841 - 216 WP_000839590.1 phage lysis protein EssD -
IHN50_RS07990 1635595..1635810 - 216 WP_000066484.1 cold shock-like protein CspB -
IHN50_RS07995 1636111..1636323 + 213 WP_000087756.1 cold shock-like protein CspF -
IHN50_RS08000 1636378..1636467 + 90 WP_120795389.1 hypothetical protein -
IHN50_RS08005 1636745..1637497 - 753 WP_001047135.1 antitermination protein -
IHN50_RS08010 1637511..1638560 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IHN50_RS08015 1638562..1638840 - 279 WP_012304870.1 hypothetical protein -
IHN50_RS08020 1638907..1639158 - 252 WP_000980994.1 hypothetical protein -
IHN50_RS08025 1639375..1639530 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IHN50_RS08030 1639602..1639889 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IHN50_RS08035 1639889..1640128 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IHN50_RS08040 1640153..1640458 + 306 WP_001326990.1 hypothetical protein -
IHN50_RS08045 1640661..1640993 + 333 WP_001301033.1 protein FlxA -
IHN50_RS08050 1641430..1641579 - 150 WP_011443592.1 hypothetical protein -
IHN50_RS08055 1641614..1641892 - 279 Protein_1574 hypothetical protein -
IHN50_RS08060 1641876..1642106 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IHN50_RS08065 1642190..1642597 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IHN50_RS08070 1642764..1642919 + 156 WP_000379575.1 DUF1391 family protein -
IHN50_RS08075 1643079..1643297 + 219 WP_001171942.1 hypothetical protein -
IHN50_RS08080 1643865..1644053 + 189 WP_000854559.1 cell division inhibition protein DicB -
IHN50_RS08085 1644050..1644241 + 192 WP_001083297.1 lysis protein YdfD -
IHN50_RS08090 1644334..1645101 + 768 Protein_1581 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625258..1658719 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T173904 WP_000323025.1 NZ_CP062245:c1639889-1639602 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T173904 NZ_CP080372:72061-72441 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT173904 WP_000534858.1 NZ_CP062245:c1640128-1639889 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT173904 NZ_CP080372:71840-72061 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References