94248

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1645346..1645872 Replicon chromosome
Accession NZ_CP026351
Organism Escherichia coli strain 5A

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C2R12_RS08635 Protein ID WP_000323025.1
Coordinates 1645346..1645633 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C2R12_RS08640 Protein ID WP_000534858.1
Coordinates 1645633..1645872 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R12_RS08595 1640370..1640585 - 216 WP_000839590.1 phage lysis protein EssD -
C2R12_RS24440 1640805..1640975 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
C2R12_RS08600 1641339..1641554 - 216 WP_000066484.1 cold shock-like protein CspB -
C2R12_RS08605 1641855..1642067 + 213 WP_000087756.1 cold shock-like protein CspF -
C2R12_RS24165 1642122..1642211 + 90 WP_120795389.1 hypothetical protein -
C2R12_RS08610 1642489..1643241 - 753 WP_001047135.1 antitermination protein -
C2R12_RS08615 1643255..1644304 - 1050 WP_001393597.1 DUF968 domain-containing protein -
C2R12_RS08620 1644306..1644584 - 279 WP_012304870.1 hypothetical protein -
C2R12_RS08625 1644651..1644902 - 252 WP_000980994.1 hypothetical protein -
C2R12_RS08630 1645119..1645274 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C2R12_RS08635 1645346..1645633 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C2R12_RS08640 1645633..1645872 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C2R12_RS08645 1645897..1646202 + 306 WP_001326990.1 hypothetical protein -
C2R12_RS08650 1646405..1646737 + 333 WP_001301033.1 protein FlxA -
C2R12_RS24170 1647174..1647323 - 150 WP_011443592.1 hypothetical protein -
C2R12_RS08660 1647358..1647636 - 279 Protein_1583 hypothetical protein -
C2R12_RS08665 1647620..1647850 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
C2R12_RS08670 1647934..1648341 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
C2R12_RS08675 1648508..1648663 + 156 WP_000379575.1 DUF1391 family protein -
C2R12_RS08685 1648823..1649041 + 219 WP_001171942.1 hypothetical protein -
C2R12_RS08695 1649609..1649797 + 189 WP_000854559.1 cell division inhibition protein DicB -
C2R12_RS08700 1649794..1649985 + 192 WP_001083297.1 lysis protein YdfD -
C2R12_RS08705 1650078..1650845 + 768 Protein_1590 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1631002..1657870 26868


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T94248 WP_000323025.1 NZ_CP026351:c1645633-1645346 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T94248 NZ_CP026351:c1645633-1645346 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT94248 WP_000534858.1 NZ_CP026351:c1645872-1645633 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT94248 NZ_CP026351:c1645872-1645633 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References