53405

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1607649..1608175 Replicon chromosome
Accession NZ_CP011113
Organism Escherichia coli strain RR1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SR35_RS08590 Protein ID WP_000323025.1
Coordinates 1607649..1607936 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SR35_RS08595 Protein ID WP_000534858.1
Coordinates 1607936..1608175 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR35_RS08550 1602673..1602888 - 216 WP_000839590.1 phage lysis protein EssD -
SR35_RS25270 1603108..1603278 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
SR35_RS08555 1603642..1603857 - 216 WP_000066484.1 cold shock-like protein CspB -
SR35_RS08560 1604158..1604370 + 213 WP_000087756.1 cold shock-like protein CspF -
SR35_RS24860 1604425..1604514 + 90 WP_120795389.1 hypothetical protein -
SR35_RS08565 1604792..1605544 - 753 WP_001047135.1 antitermination protein -
SR35_RS08570 1605558..1606607 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SR35_RS08575 1606609..1606887 - 279 WP_012304870.1 hypothetical protein -
SR35_RS08580 1606954..1607205 - 252 WP_000980994.1 hypothetical protein -
SR35_RS08585 1607422..1607577 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SR35_RS08590 1607649..1607936 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SR35_RS08595 1607936..1608175 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SR35_RS08600 1608200..1608505 + 306 WP_001326990.1 hypothetical protein -
SR35_RS08610 1608708..1609040 + 333 WP_001301033.1 protein FlxA -
SR35_RS08615 1609477..1609626 - 150 WP_011443592.1 hypothetical protein -
SR35_RS08620 1609661..1609939 - 279 Protein_1533 hypothetical protein -
SR35_RS08625 1609923..1610153 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SR35_RS08630 1610237..1610644 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SR35_RS08635 1610811..1610966 + 156 WP_000379575.1 DUF1391 family protein -
SR35_RS08645 1611126..1611344 + 219 WP_001171942.1 hypothetical protein -
SR35_RS08655 1611912..1612100 + 189 WP_000854559.1 cell division inhibition protein DicB -
SR35_RS08660 1612097..1612288 + 192 WP_001083297.1 lysis protein YdfD -
SR35_RS08665 1612381..1613148 + 768 Protein_1540 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1593305..1621509 28204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T53405 WP_000323025.1 NZ_CP011113:c1607936-1607649 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T53405 NZ_CP011113:c1607936-1607649 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT53405 WP_000534858.1 NZ_CP011113:c1608175-1607936 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT53405 NZ_CP011113:c1608175-1607936 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References