Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1607649..1608175 Replicon chromosome
Accession NZ_CP011113
Organism Escherichia coli strain RR1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SR35_RS08590 Protein ID WP_000323025.1
Coordinates 1607649..1607936 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SR35_RS08595 Protein ID WP_000534858.1
Coordinates 1607936..1608175 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR35_RS08550 1602673..1602888 - 216 WP_000839590.1 phage lysis protein EssD -
SR35_RS25270 1603108..1603278 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
SR35_RS08555 1603642..1603857 - 216 WP_000066484.1 cold shock-like protein CspB -
SR35_RS08560 1604158..1604370 + 213 WP_000087756.1 cold shock-like protein CspF -
SR35_RS24860 1604425..1604514 + 90 WP_120795389.1 hypothetical protein -
SR35_RS08565 1604792..1605544 - 753 WP_001047135.1 antitermination protein -
SR35_RS08570 1605558..1606607 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SR35_RS08575 1606609..1606887 - 279 WP_012304870.1 hypothetical protein -
SR35_RS08580 1606954..1607205 - 252 WP_000980994.1 hypothetical protein -
SR35_RS08585 1607422..1607577 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SR35_RS08590 1607649..1607936 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SR35_RS08595 1607936..1608175 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SR35_RS08600 1608200..1608505 + 306 WP_001326990.1 hypothetical protein -
SR35_RS08610 1608708..1609040 + 333 WP_001301033.1 protein FlxA -
SR35_RS08615 1609477..1609626 - 150 WP_011443592.1 hypothetical protein -
SR35_RS08620 1609661..1609939 - 279 Protein_1533 hypothetical protein -
SR35_RS08625 1609923..1610153 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SR35_RS08630 1610237..1610644 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SR35_RS08635 1610811..1610966 + 156 WP_000379575.1 DUF1391 family protein -
SR35_RS08645 1611126..1611344 + 219 WP_001171942.1 hypothetical protein -
SR35_RS08655 1611912..1612100 + 189 WP_000854559.1 cell division inhibition protein DicB -
SR35_RS08660 1612097..1612288 + 192 WP_001083297.1 lysis protein YdfD -
SR35_RS08665 1612381..1613148 + 768 Protein_1540 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1593305..1621509 28204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T53405 WP_000323025.1 NZ_CP011113:c1607936-1607649 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T53405 NZ_CP011113:c1607936-1607649 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT53405 WP_000534858.1 NZ_CP011113:c1608175-1607936 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT53405 NZ_CP011113:c1608175-1607936 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References