Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1645346..1645872 Replicon chromosome
Accession NZ_CP029238
Organism Escherichia coli strain CJ236

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DF192_RS08100 Protein ID WP_000323025.1
Coordinates 1645346..1645633 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DF192_RS08105 Protein ID WP_000534858.1
Coordinates 1645633..1645872 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DF192_RS08055 1640370..1640585 - 216 WP_000839590.1 phage lysis protein EssD -
DF192_RS22695 1640805..1640975 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DF192_RS08060 1641339..1641554 - 216 WP_000066484.1 cold shock-like protein CspB -
DF192_RS08065 1641855..1642067 + 213 WP_000087756.1 cold shock-like protein CspF -
DF192_RS08070 1642122..1642211 + 90 WP_120795389.1 hypothetical protein -
DF192_RS08075 1642489..1643241 - 753 WP_001047135.1 antitermination protein -
DF192_RS08080 1643255..1644304 - 1050 WP_001393597.1 DUF968 domain-containing protein -
DF192_RS08085 1644306..1644584 - 279 WP_012304870.1 hypothetical protein -
DF192_RS08090 1644651..1644902 - 252 WP_000980994.1 hypothetical protein -
DF192_RS08095 1645119..1645274 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DF192_RS08100 1645346..1645633 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DF192_RS08105 1645633..1645872 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DF192_RS08110 1645897..1646202 + 306 WP_001326990.1 hypothetical protein -
DF192_RS08115 1646405..1646737 + 333 WP_001301033.1 protein FlxA -
DF192_RS08120 1647174..1647323 - 150 WP_011443592.1 hypothetical protein -
DF192_RS08125 1647358..1647636 - 279 Protein_1583 hypothetical protein -
DF192_RS08130 1647620..1647850 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DF192_RS08135 1647934..1648341 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DF192_RS08140 1648508..1648663 + 156 WP_000379575.1 DUF1391 family protein -
DF192_RS08145 1648823..1649041 + 219 WP_001171942.1 hypothetical protein -
DF192_RS08155 1649609..1649797 + 189 WP_000854559.1 cell division inhibition protein DicB -
DF192_RS08160 1649794..1649985 + 192 WP_001083297.1 lysis protein YdfD -
DF192_RS08165 1650078..1650845 + 768 Protein_1590 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1631002..1664463 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T105250 WP_000323025.1 NZ_CP029238:c1645633-1645346 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T105250 NZ_CP029238:c1645633-1645346 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT105250 WP_000534858.1 NZ_CP029238:c1645872-1645633 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT105250 NZ_CP029238:c1645872-1645633 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References