Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1634263..1634789 Replicon chromosome
Accession NZ_CP040663
Organism Escherichia coli strain EK2009

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FE383_RS08050 Protein ID WP_000323025.1
Coordinates 1634263..1634550 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FE383_RS08055 Protein ID WP_000534858.1
Coordinates 1634550..1634789 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE383_RS08005 1629287..1629502 - 216 WP_000839590.1 phage lysis protein EssD -
FE383_RS22480 1629722..1629892 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
FE383_RS08010 1630256..1630471 - 216 WP_000066484.1 cold shock-like protein CspB -
FE383_RS08015 1630772..1630984 + 213 WP_000087756.1 cold shock-like protein CspF -
FE383_RS08020 1631039..1631128 + 90 WP_120795389.1 hypothetical protein -
FE383_RS08025 1631406..1632158 - 753 WP_001047135.1 antitermination protein -
FE383_RS08030 1632172..1633221 - 1050 WP_001265198.1 DUF968 domain-containing protein -
FE383_RS08035 1633223..1633501 - 279 WP_012304870.1 hypothetical protein -
FE383_RS08040 1633568..1633819 - 252 WP_000980994.1 hypothetical protein -
FE383_RS08045 1634036..1634191 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FE383_RS08050 1634263..1634550 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FE383_RS08055 1634550..1634789 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FE383_RS08060 1634814..1635119 + 306 WP_001326990.1 hypothetical protein -
FE383_RS08065 1635322..1635654 + 333 WP_001301033.1 protein FlxA -
FE383_RS08070 1636091..1636240 - 150 WP_011443592.1 hypothetical protein -
FE383_RS08075 1636275..1636553 - 279 Protein_1572 hypothetical protein -
FE383_RS08080 1636537..1636767 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FE383_RS08085 1636851..1637258 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FE383_RS08090 1637425..1637580 + 156 WP_000379575.1 DUF1391 family protein -
FE383_RS08095 1637740..1637958 + 219 WP_001171942.1 hypothetical protein -
FE383_RS08105 1638526..1638714 + 189 WP_000854559.1 cell division inhibition protein DicB -
FE383_RS08110 1638711..1638902 + 192 WP_001083297.1 lysis protein YdfD -
FE383_RS08115 1638995..1639762 + 768 Protein_1579 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1619919..1653380 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T126352 WP_000323025.1 NZ_CP040663:c1634550-1634263 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T126352 NZ_CP040663:c1634550-1634263 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT126352 WP_000534858.1 NZ_CP040663:c1634789-1634550 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT126352 NZ_CP040663:c1634789-1634550 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References