Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3774271..3774797 Replicon chromosome
Accession NZ_CP030240
Organism Escherichia coli strain ER1709

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DQ225_RS18340 Protein ID WP_000323025.1
Coordinates 3774271..3774558 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DQ225_RS18345 Protein ID WP_000534858.1
Coordinates 3774558..3774797 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQ225_RS18295 3769296..3769511 - 216 WP_000839590.1 phage lysis protein EssD -
DQ225_RS22775 3769731..3769901 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DQ225_RS18300 3770265..3770480 - 216 WP_000066484.1 cold shock-like protein CspB -
DQ225_RS18305 3770781..3770993 + 213 WP_000087756.1 cold shock-like protein CspF -
DQ225_RS18310 3771048..3771137 + 90 WP_120795389.1 hypothetical protein -
DQ225_RS18315 3771414..3772166 - 753 WP_001047135.1 antitermination protein -
DQ225_RS18320 3772180..3773229 - 1050 WP_001265198.1 DUF968 domain-containing protein -
DQ225_RS18325 3773231..3773509 - 279 WP_012304870.1 hypothetical protein -
DQ225_RS18330 3773576..3773827 - 252 WP_000980994.1 hypothetical protein -
DQ225_RS18335 3774044..3774199 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DQ225_RS18340 3774271..3774558 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DQ225_RS18345 3774558..3774797 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DQ225_RS18350 3774822..3775127 + 306 WP_001326990.1 hypothetical protein -
DQ225_RS18355 3775330..3775662 + 333 WP_001301033.1 protein FlxA -
DQ225_RS18360 3776099..3776248 - 150 WP_011443592.1 hypothetical protein -
DQ225_RS18365 3776283..3776561 - 279 Protein_3555 hypothetical protein -
DQ225_RS18370 3776545..3776775 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DQ225_RS18375 3776859..3777266 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DQ225_RS18380 3777433..3777588 + 156 WP_000379575.1 DUF1391 family protein -
DQ225_RS18385 3777748..3777966 + 219 WP_001171942.1 hypothetical protein -
DQ225_RS18395 3778534..3778722 + 189 WP_000854559.1 cell division inhibition protein DicB -
DQ225_RS18400 3778719..3778910 + 192 WP_001083297.1 lysis protein YdfD -
DQ225_RS18405 3779003..3779770 + 768 Protein_3562 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3759929..3794723 34794


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T107391 WP_000323025.1 NZ_CP030240:c3774558-3774271 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T107391 NZ_CP030240:c3774558-3774271 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT107391 WP_000534858.1 NZ_CP030240:c3774797-3774558 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT107391 NZ_CP030240:c3774797-3774558 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References