Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639601..1640127 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IVL01_RS08030 Protein ID WP_000323025.1
Coordinates 1639601..1639888 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IVL01_RS08035 Protein ID WP_000534858.1
Coordinates 1639888..1640127 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS07985 1634625..1634840 - 216 WP_000839590.1 phage lysis protein EssD -
IVL01_RS07990 1635594..1635809 - 216 WP_000066484.1 cold shock-like protein CspB -
IVL01_RS07995 1636110..1636322 + 213 WP_000087756.1 cold shock-like protein CspF -
IVL01_RS08000 1636377..1636466 + 90 WP_120795389.1 hypothetical protein -
IVL01_RS08005 1636744..1637496 - 753 WP_001047135.1 antitermination protein -
IVL01_RS08010 1637510..1638559 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IVL01_RS08015 1638561..1638839 - 279 WP_012304870.1 hypothetical protein -
IVL01_RS08020 1638906..1639157 - 252 WP_000980994.1 hypothetical protein -
IVL01_RS08025 1639374..1639529 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IVL01_RS08030 1639601..1639888 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IVL01_RS08035 1639888..1640127 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IVL01_RS08040 1640152..1640457 + 306 WP_001326990.1 hypothetical protein -
IVL01_RS08045 1640660..1640992 + 333 WP_001301033.1 protein FlxA -
IVL01_RS08050 1641429..1641578 - 150 WP_011443592.1 hypothetical protein -
IVL01_RS08055 1641613..1641891 - 279 Protein_1574 hypothetical protein -
IVL01_RS08060 1641875..1642105 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IVL01_RS08065 1642189..1642596 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IVL01_RS08070 1642763..1642918 + 156 WP_000379575.1 DUF1391 family protein -
IVL01_RS08075 1643078..1643296 + 219 WP_001171942.1 hypothetical protein -
IVL01_RS08080 1643864..1644052 + 189 WP_000854559.1 cell division inhibition protein DicB -
IVL01_RS08085 1644049..1644240 + 192 WP_001083297.1 lysis protein YdfD -
IVL01_RS08090 1644333..1645100 + 768 Protein_1581 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625257..1658718 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T181513 WP_000323025.1 NZ_CP064680:c1639888-1639601 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T181513 NZ_CP085906:326424-326765 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTGTGGATTGATTTTGATCCTTCGATAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAGTTTAATCGTAAAACAATGTTTTCGGTCATATGTCCGATTA
CTTCCACGATAAAGAATTTACCGACACGTTACTCATTACCAGAAGAGCTAGATATAAAAGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTTAAGGAACGAAGACTCAAAAAAATAGAAAACCTGCCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAATATATGTTTTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT181513 WP_000534858.1 NZ_CP064680:c1640127-1639888 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT181513 NZ_CP085906:326194-326424 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTTACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTATATTCTGACGATGGGACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GAGAAGAGGCCGAATATTATGAAAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAAGGTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References