Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2099231..2099757 Replicon chromosome
Accession NZ_CP034595
Organism Escherichia coli strain WCHEC035053S1G0

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DK245_RS10300 Protein ID WP_000323025.1
Coordinates 2099470..2099757 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DK245_RS10295 Protein ID WP_000534858.1
Coordinates 2099231..2099470 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK245_RS10235 2094258..2095025 - 768 Protein_1981 exonuclease -
DK245_RS10240 2095118..2095309 - 192 WP_001083297.1 lysis protein YdfD -
DK245_RS10245 2095306..2095494 - 189 WP_000854559.1 cell division inhibition protein DicB -
DK245_RS10255 2096062..2096280 - 219 WP_001171942.1 hypothetical protein -
DK245_RS10260 2096440..2096595 - 156 WP_000379575.1 DUF1391 family protein -
DK245_RS10265 2096762..2097169 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DK245_RS10270 2097253..2097483 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
DK245_RS10275 2097467..2097745 + 279 Protein_1988 hypothetical protein -
DK245_RS10280 2097780..2097929 + 150 WP_011443592.1 hypothetical protein -
DK245_RS10285 2098366..2098698 - 333 WP_001301033.1 protein FlxA -
DK245_RS10290 2098901..2099206 - 306 WP_001326990.1 hypothetical protein -
DK245_RS10295 2099231..2099470 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DK245_RS10300 2099470..2099757 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DK245_RS10305 2099829..2099984 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DK245_RS10310 2100201..2100452 + 252 WP_000980994.1 hypothetical protein -
DK245_RS10315 2100519..2100797 + 279 WP_012304870.1 hypothetical protein -
DK245_RS10320 2100799..2101848 + 1050 WP_001265198.1 DUF968 domain-containing protein -
DK245_RS10325 2101862..2102614 + 753 WP_001047135.1 antitermination protein -
DK245_RS10330 2102892..2102981 - 90 WP_120795389.1 hypothetical protein -
DK245_RS10335 2103036..2103248 - 213 WP_000087756.1 cold shock-like protein CspF -
DK245_RS10340 2103549..2103764 + 216 WP_000066484.1 cold shock-like protein CspB -
DK245_RS23060 2104128..2104298 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
DK245_RS10345 2104518..2104733 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2080640..2114101 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T116275 WP_000323025.1 NZ_CP034595:2099470-2099757 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T116275 NZ_CP034595:2099470-2099757 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT116275 WP_000534858.1 NZ_CP034595:2099231-2099470 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT116275 NZ_CP034595:2099231-2099470 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References