116275

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2099231..2099757 Replicon chromosome
Accession NZ_CP034595
Organism Escherichia coli strain WCHEC035053S1G0

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DK245_RS10300 Protein ID WP_000323025.1
Coordinates 2099470..2099757 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DK245_RS10295 Protein ID WP_000534858.1
Coordinates 2099231..2099470 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK245_RS10235 2094258..2095025 - 768 Protein_1981 exonuclease -
DK245_RS10240 2095118..2095309 - 192 WP_001083297.1 lysis protein YdfD -
DK245_RS10245 2095306..2095494 - 189 WP_000854559.1 cell division inhibition protein DicB -
DK245_RS10255 2096062..2096280 - 219 WP_001171942.1 hypothetical protein -
DK245_RS10260 2096440..2096595 - 156 WP_000379575.1 DUF1391 family protein -
DK245_RS10265 2096762..2097169 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DK245_RS10270 2097253..2097483 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
DK245_RS10275 2097467..2097745 + 279 Protein_1988 hypothetical protein -
DK245_RS10280 2097780..2097929 + 150 WP_011443592.1 hypothetical protein -
DK245_RS10285 2098366..2098698 - 333 WP_001301033.1 protein FlxA -
DK245_RS10290 2098901..2099206 - 306 WP_001326990.1 hypothetical protein -
DK245_RS10295 2099231..2099470 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DK245_RS10300 2099470..2099757 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DK245_RS10305 2099829..2099984 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DK245_RS10310 2100201..2100452 + 252 WP_000980994.1 hypothetical protein -
DK245_RS10315 2100519..2100797 + 279 WP_012304870.1 hypothetical protein -
DK245_RS10320 2100799..2101848 + 1050 WP_001265198.1 DUF968 domain-containing protein -
DK245_RS10325 2101862..2102614 + 753 WP_001047135.1 antitermination protein -
DK245_RS10330 2102892..2102981 - 90 WP_120795389.1 hypothetical protein -
DK245_RS10335 2103036..2103248 - 213 WP_000087756.1 cold shock-like protein CspF -
DK245_RS10340 2103549..2103764 + 216 WP_000066484.1 cold shock-like protein CspB -
DK245_RS23060 2104128..2104298 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
DK245_RS10345 2104518..2104733 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2080640..2114101 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T116275 WP_000323025.1 NZ_CP034595:2099470-2099757 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T116275 NZ_CP034595:2099470-2099757 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT116275 WP_000534858.1 NZ_CP034595:2099231-2099470 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT116275 NZ_CP034595:2099231-2099470 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References