Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1624916..1625442 Replicon chromosome
Accession NZ_CP012127
Organism Escherichia coli strain DH1Ec169

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ADZ28_RS08045 Protein ID WP_000323025.1
Coordinates 1624916..1625203 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ADZ28_RS08050 Protein ID WP_000534858.1
Coordinates 1625203..1625442 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ28_RS08005 1619940..1620155 - 216 WP_000839590.1 phage lysis protein EssD -
ADZ28_RS08010 1620909..1621124 - 216 WP_000066484.1 cold shock-like protein CspB -
ADZ28_RS08015 1621425..1621637 + 213 WP_000087756.1 cold shock-like protein CspF -
ADZ28_RS25355 1621692..1621781 + 90 WP_120795389.1 hypothetical protein -
ADZ28_RS08020 1622059..1622811 - 753 WP_001047135.1 antitermination protein -
ADZ28_RS08025 1622825..1623874 - 1050 WP_001265198.1 DUF968 domain-containing protein -
ADZ28_RS08030 1623876..1624154 - 279 WP_012304870.1 hypothetical protein -
ADZ28_RS08035 1624221..1624472 - 252 WP_000980994.1 hypothetical protein -
ADZ28_RS08040 1624689..1624844 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ADZ28_RS08045 1624916..1625203 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ADZ28_RS08050 1625203..1625442 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ADZ28_RS23550 1625467..1625772 + 306 WP_001326990.1 hypothetical protein -
ADZ28_RS08055 1625975..1626307 + 333 WP_001301033.1 protein FlxA -
ADZ28_RS22785 1626744..1626893 - 150 WP_011443592.1 hypothetical protein -
ADZ28_RS08060 1626928..1627206 - 279 Protein_1547 hypothetical protein -
ADZ28_RS08065 1627190..1627420 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
ADZ28_RS08070 1627504..1627911 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ADZ28_RS08075 1628078..1628233 + 156 WP_000379575.1 DUF1391 family protein -
ADZ28_RS08080 1628393..1628611 + 219 WP_001171942.1 hypothetical protein -
ADZ28_RS08090 1629179..1629367 + 189 WP_000854559.1 cell division inhibition protein DicB -
ADZ28_RS08095 1629364..1629555 + 192 WP_001083297.1 lysis protein YdfD -
ADZ28_RS23565 1629648..1630415 + 768 Protein_1554 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1610572..1644033 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T55926 WP_000323025.1 NZ_CP012127:c1625203-1624916 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T55926 NZ_CP012127:c1625203-1624916 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT55926 WP_000534858.1 NZ_CP012127:c1625442-1625203 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT55926 NZ_CP012127:c1625442-1625203 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References