210463

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2499973..2500499 Replicon chromosome
Accession NZ_CP079991
Organism Escherichia coli strain KMS2005

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KYK86_RS12150 Protein ID WP_000323025.1
Coordinates 2499973..2500260 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KYK86_RS12155 Protein ID WP_000534858.1
Coordinates 2500260..2500499 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK86_RS12100 (2494997) 2494997..2495212 - 216 WP_000839590.1 phage lysis protein EssD -
KYK86_RS12105 (2495432) 2495432..2495602 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
KYK86_RS12110 (2495966) 2495966..2496181 - 216 WP_000066484.1 cold shock-like protein CspB -
KYK86_RS12115 (2496482) 2496482..2496694 + 213 WP_000087756.1 cold shock-like protein CspF -
KYK86_RS12120 (2496749) 2496749..2496838 + 90 WP_120795389.1 hypothetical protein -
KYK86_RS12125 (2497116) 2497116..2497868 - 753 WP_001047135.1 antitermination protein -
KYK86_RS12130 (2497882) 2497882..2498931 - 1050 WP_001393597.1 DUF968 domain-containing protein -
KYK86_RS12135 (2498933) 2498933..2499211 - 279 WP_012304870.1 hypothetical protein -
KYK86_RS12140 (2499278) 2499278..2499529 - 252 WP_000980994.1 protein Rem -
KYK86_RS12145 (2499746) 2499746..2499901 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
KYK86_RS12150 (2499973) 2499973..2500260 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KYK86_RS12155 (2500260) 2500260..2500499 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KYK86_RS12160 (2500524) 2500524..2500829 + 306 WP_001326990.1 protein YdfV -
KYK86_RS12165 (2501032) 2501032..2501364 + 333 WP_001301033.1 FlxA-like family protein -
KYK86_RS12170 (2501801) 2501801..2501950 - 150 WP_011443592.1 protein YdfW -
KYK86_RS12175 (2501985) 2501985..2502263 - 279 Protein_2391 protein YdfX -
KYK86_RS12180 (2502247) 2502247..2502477 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
KYK86_RS12185 (2502561) 2502561..2502968 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
KYK86_RS12190 (2503135) 2503135..2503290 + 156 WP_000379575.1 DUF1391 family protein -
KYK86_RS22955 (2503292) 2503292..2503420 + 129 WP_000344964.1 protein YdfB -
KYK86_RS12195 (2503450) 2503450..2503668 + 219 WP_001171942.1 protein YdfC -
KYK86_RS12200 (2504236) 2504236..2504424 + 189 WP_000854559.1 cell division inhibition protein DicB -
KYK86_RS12205 (2504421) 2504421..2504612 + 192 WP_001083297.1 lysis protein YdfD -
KYK86_RS12210 (2504705) 2504705..2505472 + 768 Protein_2399 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2485629..2519090 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T210463 WP_000323025.1 NZ_CP079991:c2500260-2499973 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T210463 NZ_CP104523:c3398949-3398551 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT210463 WP_000534858.1 NZ_CP079991:c2500499-2500260 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT210463 NZ_CP104523:c3399176-3398949 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References