Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2388917..2389443 Replicon chromosome
Accession NZ_CP010441
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH03_RS11805 Protein ID WP_000323025.1
Coordinates 2388917..2389204 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH03_RS11810 Protein ID WP_000534858.1
Coordinates 2389204..2389443 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH03_RS11765 2383941..2384156 - 216 WP_000839590.1 phage lysis protein EssD -
SH03_RS11770 2384910..2385125 - 216 WP_000066484.1 cold shock-like protein CspB -
SH03_RS11775 2385426..2385638 + 213 WP_000087756.1 cold shock-like protein CspF -
SH03_RS25580 2385693..2385782 + 90 WP_120795389.1 hypothetical protein -
SH03_RS11780 2386060..2386812 - 753 WP_001047135.1 antitermination protein -
SH03_RS11785 2386826..2387875 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH03_RS11790 2387877..2388155 - 279 WP_012304870.1 hypothetical protein -
SH03_RS11795 2388222..2388473 - 252 WP_000980994.1 hypothetical protein -
SH03_RS11800 2388690..2388845 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH03_RS11805 2388917..2389204 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH03_RS11810 2389204..2389443 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH03_RS24010 2389468..2389773 + 306 WP_001326990.1 hypothetical protein -
SH03_RS11815 2389976..2390308 + 333 WP_001301033.1 protein FlxA -
SH03_RS22980 2390745..2390894 - 150 WP_011443592.1 hypothetical protein -
SH03_RS11820 2390929..2391207 - 279 Protein_2226 hypothetical protein -
SH03_RS11825 2391191..2391421 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH03_RS11830 2391505..2391912 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH03_RS11835 2392079..2392234 + 156 WP_000379575.1 DUF1391 family protein -
SH03_RS11840 2392394..2392612 + 219 WP_001171942.1 hypothetical protein -
SH03_RS11850 2393180..2393368 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH03_RS11855 2393365..2393556 + 192 WP_038432577.1 lysis protein YdfD -
SH03_RS24025 2393649..2394416 + 768 Protein_2233 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2374573..2408034 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52548 WP_000323025.1 NZ_CP010441:c2389204-2388917 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52548 NZ_CP010441:c2389204-2388917 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52548 WP_000534858.1 NZ_CP010441:c2389443-2389204 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52548 NZ_CP010441:c2389443-2389204 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References