52548

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2388917..2389443 Replicon chromosome
Accession NZ_CP010441
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH03_RS11805 Protein ID WP_000323025.1
Coordinates 2388917..2389204 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH03_RS11810 Protein ID WP_000534858.1
Coordinates 2389204..2389443 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH03_RS11765 2383941..2384156 - 216 WP_000839590.1 phage lysis protein EssD -
SH03_RS11770 2384910..2385125 - 216 WP_000066484.1 cold shock-like protein CspB -
SH03_RS11775 2385426..2385638 + 213 WP_000087756.1 cold shock-like protein CspF -
SH03_RS25580 2385693..2385782 + 90 WP_120795389.1 hypothetical protein -
SH03_RS11780 2386060..2386812 - 753 WP_001047135.1 antitermination protein -
SH03_RS11785 2386826..2387875 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH03_RS11790 2387877..2388155 - 279 WP_012304870.1 hypothetical protein -
SH03_RS11795 2388222..2388473 - 252 WP_000980994.1 hypothetical protein -
SH03_RS11800 2388690..2388845 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH03_RS11805 2388917..2389204 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH03_RS11810 2389204..2389443 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH03_RS24010 2389468..2389773 + 306 WP_001326990.1 hypothetical protein -
SH03_RS11815 2389976..2390308 + 333 WP_001301033.1 protein FlxA -
SH03_RS22980 2390745..2390894 - 150 WP_011443592.1 hypothetical protein -
SH03_RS11820 2390929..2391207 - 279 Protein_2226 hypothetical protein -
SH03_RS11825 2391191..2391421 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH03_RS11830 2391505..2391912 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH03_RS11835 2392079..2392234 + 156 WP_000379575.1 DUF1391 family protein -
SH03_RS11840 2392394..2392612 + 219 WP_001171942.1 hypothetical protein -
SH03_RS11850 2393180..2393368 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH03_RS11855 2393365..2393556 + 192 WP_038432577.1 lysis protein YdfD -
SH03_RS24025 2393649..2394416 + 768 Protein_2233 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2374573..2408034 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52548 WP_000323025.1 NZ_CP010441:c2389204-2388917 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52548 NZ_CP010441:c2389204-2388917 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52548 WP_000534858.1 NZ_CP010441:c2389443-2389204 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52548 NZ_CP010441:c2389443-2389204 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References