285435

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1594243..1594769 Replicon chromosome
Accession NZ_LM995446
Organism Escherichia coli strain K-12 substr. RV308

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECRV308_RS07955 Protein ID WP_000323025.1
Coordinates 1594243..1594530 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECRV308_RS07960 Protein ID WP_000534858.1
Coordinates 1594530..1594769 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRV308_RS07915 1589267..1589482 - 216 WP_000839590.1 phage lysis protein EssD -
ECRV308_RS07920 1590236..1590451 - 216 WP_000066484.1 cold shock-like protein CspB -
ECRV308_RS07925 1590752..1590964 + 213 WP_000087756.1 cold shock-like protein CspF -
ECRV308_RS25340 1591019..1591108 + 90 WP_120795389.1 hypothetical protein -
ECRV308_RS07930 1591386..1592138 - 753 WP_001047135.1 antitermination protein -
ECRV308_RS07935 1592152..1593201 - 1050 WP_001265198.1 DUF968 domain-containing protein -
ECRV308_RS07940 1593203..1593481 - 279 WP_012304870.1 hypothetical protein -
ECRV308_RS07945 1593548..1593799 - 252 WP_000980994.1 hypothetical protein -
ECRV308_RS07950 1594016..1594171 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ECRV308_RS07955 1594243..1594530 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECRV308_RS07960 1594530..1594769 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECRV308_RS23530 1594794..1595099 + 306 WP_001326990.1 hypothetical protein -
ECRV308_RS07965 1595302..1595634 + 333 WP_001301033.1 protein FlxA -
ECRV308_RS22835 1596071..1596220 - 150 WP_011443592.1 hypothetical protein -
ECRV308_RS07970 1596255..1596533 - 279 Protein_1521 hypothetical protein -
ECRV308_RS07975 1596517..1596747 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
ECRV308_RS07980 1596831..1597238 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ECRV308_RS07985 1597405..1597560 + 156 WP_000379575.1 DUF1391 family protein -
ECRV308_RS07990 1597720..1597938 + 219 WP_001171942.1 hypothetical protein -
ECRV308_RS08000 1598506..1598694 + 189 WP_000854559.1 cell division inhibition protein DicB -
ECRV308_RS08005 1598691..1598882 + 192 WP_001083297.1 lysis protein YdfD -
ECRV308_RS23545 1598975..1599742 + 768 Protein_1528 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1579899..1613360 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T285435 WP_000323025.1 NZ_LM995446:c1594530-1594243 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT285435 WP_000534858.1 NZ_LM995446:c1594769-1594530 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References