Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2276645..2277171 Replicon chromosome
Accession NZ_CP031214
Organism Escherichia coli strain C600

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DK885_RS11725 Protein ID WP_000323025.1
Coordinates 2276884..2277171 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DK885_RS11720 Protein ID WP_000534858.1
Coordinates 2276645..2276884 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK885_RS11655 2271672..2272439 - 768 Protein_2135 exonuclease -
DK885_RS11660 2272532..2272723 - 192 WP_001083297.1 lysis protein YdfD -
DK885_RS11665 2272720..2272908 - 189 WP_000854559.1 cell division inhibition protein DicB -
DK885_RS11675 2273476..2273694 - 219 WP_001171942.1 hypothetical protein -
DK885_RS11685 2273854..2274009 - 156 WP_000379575.1 DUF1391 family protein -
DK885_RS11690 2274176..2274583 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DK885_RS11695 2274667..2274897 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
DK885_RS11700 2274881..2275159 + 279 Protein_2142 hypothetical protein -
DK885_RS23905 2275194..2275343 + 150 WP_011443592.1 hypothetical protein -
DK885_RS11710 2275780..2276112 - 333 WP_001301033.1 protein FlxA -
DK885_RS11715 2276315..2276620 - 306 WP_001326990.1 hypothetical protein -
DK885_RS11720 2276645..2276884 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DK885_RS11725 2276884..2277171 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DK885_RS11730 2277243..2277398 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DK885_RS11735 2277615..2277866 + 252 WP_000980994.1 hypothetical protein -
DK885_RS11740 2277933..2278211 + 279 WP_012304870.1 hypothetical protein -
DK885_RS11745 2278213..2279262 + 1050 WP_001265198.1 DUF968 domain-containing protein -
DK885_RS11750 2279276..2280028 + 753 WP_001047135.1 antitermination protein -
DK885_RS23910 2280306..2280395 - 90 WP_120795389.1 hypothetical protein -
DK885_RS11755 2280450..2280662 - 213 WP_000087756.1 cold shock-like protein CspF -
DK885_RS11760 2280963..2281178 + 216 WP_000066484.1 cold shock-like protein CspB -
DK885_RS24245 2281542..2281712 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
DK885_RS11765 2281932..2282147 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2258054..2298412 40358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T108583 WP_000323025.1 NZ_CP031214:2276884-2277171 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T108583 NZ_CP031214:2276884-2277171 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT108583 WP_000534858.1 NZ_CP031214:2276645-2276884 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT108583 NZ_CP031214:2276645-2276884 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References