Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1645345..1645871 Replicon chromosome
Accession NZ_CP026343
Organism Escherichia coli strain 9A

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C2R20_RS08635 Protein ID WP_000323025.1
Coordinates 1645345..1645632 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C2R20_RS08640 Protein ID WP_000534858.1
Coordinates 1645632..1645871 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R20_RS08595 1640369..1640584 - 216 WP_000839590.1 phage lysis protein EssD -
C2R20_RS24440 1640804..1640974 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
C2R20_RS08600 1641338..1641553 - 216 WP_000066484.1 cold shock-like protein CspB -
C2R20_RS08605 1641854..1642066 + 213 WP_000087756.1 cold shock-like protein CspF -
C2R20_RS24165 1642121..1642210 + 90 WP_120795389.1 hypothetical protein -
C2R20_RS08610 1642488..1643240 - 753 WP_001047135.1 antitermination protein -
C2R20_RS08615 1643254..1644303 - 1050 WP_001393597.1 DUF968 domain-containing protein -
C2R20_RS08620 1644305..1644583 - 279 WP_012304870.1 hypothetical protein -
C2R20_RS08625 1644650..1644901 - 252 WP_000980994.1 hypothetical protein -
C2R20_RS08630 1645118..1645273 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C2R20_RS08635 1645345..1645632 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C2R20_RS08640 1645632..1645871 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C2R20_RS08645 1645896..1646201 + 306 WP_001326990.1 hypothetical protein -
C2R20_RS08650 1646404..1646736 + 333 WP_001301033.1 protein FlxA -
C2R20_RS24170 1647173..1647322 - 150 WP_011443592.1 hypothetical protein -
C2R20_RS08660 1647357..1647635 - 279 Protein_1583 hypothetical protein -
C2R20_RS08665 1647619..1647849 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
C2R20_RS08670 1647933..1648340 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
C2R20_RS08675 1648507..1648662 + 156 WP_000379575.1 DUF1391 family protein -
C2R20_RS08685 1648822..1649040 + 219 WP_001171942.1 hypothetical protein -
C2R20_RS08695 1649608..1649796 + 189 WP_000854559.1 cell division inhibition protein DicB -
C2R20_RS08700 1649793..1649984 + 192 WP_001083297.1 lysis protein YdfD -
C2R20_RS08705 1650077..1650844 + 768 Protein_1590 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1631001..1664462 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T93904 WP_000323025.1 NZ_CP026343:c1645632-1645345 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T93904 NZ_CP026343:c1645632-1645345 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT93904 WP_000534858.1 NZ_CP026343:c1645871-1645632 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT93904 NZ_CP026343:c1645871-1645632 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References