Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2240054..2240580 Replicon chromosome
Accession NZ_CP067994
Organism Escherichia coli strain CV601

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JJT18_RS10765 Protein ID WP_000323025.1
Coordinates 2240293..2240580 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JJT18_RS10760 Protein ID WP_000534858.1
Coordinates 2240054..2240293 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJT18_RS10705 2235081..2235848 - 768 Protein_2089 exonuclease -
JJT18_RS10710 2235941..2236132 - 192 WP_001083297.1 lysis protein YdfD -
JJT18_RS10715 2236129..2236317 - 189 WP_000854559.1 cell division inhibition protein DicB -
JJT18_RS10720 2236885..2237103 - 219 WP_001171942.1 hypothetical protein -
JJT18_RS10725 2237263..2237418 - 156 WP_000379575.1 DUF1391 family protein -
JJT18_RS10730 2237585..2237992 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
JJT18_RS10735 2238076..2238306 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
JJT18_RS10740 2238290..2238568 + 279 Protein_2096 hypothetical protein -
JJT18_RS10745 2238603..2238752 + 150 WP_011443592.1 hypothetical protein -
JJT18_RS10750 2239189..2239521 - 333 WP_001301033.1 protein FlxA -
JJT18_RS10755 2239724..2240029 - 306 WP_001326990.1 hypothetical protein -
JJT18_RS10760 2240054..2240293 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JJT18_RS10765 2240293..2240580 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JJT18_RS10770 2240652..2240807 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JJT18_RS10775 2241024..2241275 + 252 WP_000980994.1 hypothetical protein -
JJT18_RS10780 2241342..2241620 + 279 WP_012304870.1 hypothetical protein -
JJT18_RS10785 2241622..2242671 + 1050 WP_001265198.1 DUF968 domain-containing protein -
JJT18_RS10790 2242685..2243437 + 753 WP_001047135.1 antitermination protein -
JJT18_RS10795 2243715..2243804 - 90 WP_120795389.1 hypothetical protein -
JJT18_RS10800 2243859..2244071 - 213 WP_000087756.1 cold shock-like protein CspF -
JJT18_RS10805 2244372..2244587 + 216 WP_000066484.1 cold shock-like protein CspB -
JJT18_RS10810 2245341..2245556 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2221463..2254924 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T186401 WP_000323025.1 NZ_CP067994:2240293-2240580 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T186401 NZ_CP089415:c2865916-2865665 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT186401 WP_000534858.1 NZ_CP067994:2240054-2240293 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT186401 NZ_CP089415:c2866221-2865943 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References