Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1645351..1645877 Replicon chromosome
Accession NZ_CP032679
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D7U33_RS08030 Protein ID WP_000323025.1
Coordinates 1645351..1645638 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D7U33_RS08035 Protein ID WP_000534858.1
Coordinates 1645638..1645877 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7U33_RS07985 1640375..1640590 - 216 WP_000839590.1 phage lysis protein EssD -
D7U33_RS07990 1641344..1641559 - 216 WP_000066484.1 cold shock-like protein CspB -
D7U33_RS07995 1641860..1642072 + 213 WP_000087756.1 cold shock-like protein CspF -
D7U33_RS08000 1642127..1642216 + 90 WP_120795389.1 hypothetical protein -
D7U33_RS08005 1642494..1643246 - 753 WP_001047135.1 antitermination protein -
D7U33_RS08010 1643260..1644309 - 1050 WP_001393597.1 DUF968 domain-containing protein -
D7U33_RS08015 1644311..1644589 - 279 WP_012304870.1 hypothetical protein -
D7U33_RS08020 1644656..1644907 - 252 WP_000980994.1 hypothetical protein -
D7U33_RS08025 1645124..1645279 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
D7U33_RS08030 1645351..1645638 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D7U33_RS08035 1645638..1645877 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D7U33_RS08040 1645902..1646207 + 306 WP_001326990.1 hypothetical protein -
D7U33_RS08045 1646410..1646742 + 333 WP_001301033.1 protein FlxA -
D7U33_RS08050 1647179..1647328 - 150 WP_011443592.1 hypothetical protein -
D7U33_RS08055 1647363..1647641 - 279 Protein_1574 hypothetical protein -
D7U33_RS08060 1647625..1647855 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
D7U33_RS08065 1647939..1648346 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
D7U33_RS08070 1648513..1648668 + 156 WP_000379575.1 DUF1391 family protein -
D7U33_RS08075 1648828..1649046 + 219 WP_001171942.1 hypothetical protein -
D7U33_RS08080 1649614..1649802 + 189 WP_000854559.1 cell division inhibition protein DicB -
D7U33_RS08085 1649799..1649990 + 192 WP_001083297.1 lysis protein YdfD -
D7U33_RS08090 1650083..1650850 + 768 Protein_1581 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1631007..1664468 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T112107 WP_000323025.1 NZ_CP032679:c1645638-1645351 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T112107 NZ_CP032679:c1645638-1645351 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT112107 WP_000534858.1 NZ_CP032679:c1645877-1645638 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT112107 NZ_CP032679:c1645877-1645638 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References