112107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1645351..1645877 Replicon chromosome
Accession NZ_CP032679
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D7U33_RS08030 Protein ID WP_000323025.1
Coordinates 1645351..1645638 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D7U33_RS08035 Protein ID WP_000534858.1
Coordinates 1645638..1645877 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7U33_RS07985 1640375..1640590 - 216 WP_000839590.1 phage lysis protein EssD -
D7U33_RS07990 1641344..1641559 - 216 WP_000066484.1 cold shock-like protein CspB -
D7U33_RS07995 1641860..1642072 + 213 WP_000087756.1 cold shock-like protein CspF -
D7U33_RS08000 1642127..1642216 + 90 WP_120795389.1 hypothetical protein -
D7U33_RS08005 1642494..1643246 - 753 WP_001047135.1 antitermination protein -
D7U33_RS08010 1643260..1644309 - 1050 WP_001393597.1 DUF968 domain-containing protein -
D7U33_RS08015 1644311..1644589 - 279 WP_012304870.1 hypothetical protein -
D7U33_RS08020 1644656..1644907 - 252 WP_000980994.1 hypothetical protein -
D7U33_RS08025 1645124..1645279 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
D7U33_RS08030 1645351..1645638 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D7U33_RS08035 1645638..1645877 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D7U33_RS08040 1645902..1646207 + 306 WP_001326990.1 hypothetical protein -
D7U33_RS08045 1646410..1646742 + 333 WP_001301033.1 protein FlxA -
D7U33_RS08050 1647179..1647328 - 150 WP_011443592.1 hypothetical protein -
D7U33_RS08055 1647363..1647641 - 279 Protein_1574 hypothetical protein -
D7U33_RS08060 1647625..1647855 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
D7U33_RS08065 1647939..1648346 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
D7U33_RS08070 1648513..1648668 + 156 WP_000379575.1 DUF1391 family protein -
D7U33_RS08075 1648828..1649046 + 219 WP_001171942.1 hypothetical protein -
D7U33_RS08080 1649614..1649802 + 189 WP_000854559.1 cell division inhibition protein DicB -
D7U33_RS08085 1649799..1649990 + 192 WP_001083297.1 lysis protein YdfD -
D7U33_RS08090 1650083..1650850 + 768 Protein_1581 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1631007..1664468 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T112107 WP_000323025.1 NZ_CP032679:c1645638-1645351 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T112107 NZ_CP032679:c1645638-1645351 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT112107 WP_000534858.1 NZ_CP032679:c1645877-1645638 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT112107 NZ_CP032679:c1645877-1645638 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References