Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2349722..2350248 Replicon chromosome
Accession NZ_CP042845
Organism Escherichia coli strain JME65

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FVE21_RS11450 Protein ID WP_000323025.1
Coordinates 2349722..2350009 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FVE21_RS11455 Protein ID WP_000534858.1
Coordinates 2350009..2350248 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE21_RS11405 2344746..2344961 - 216 WP_000839590.1 phage lysis protein EssD -
FVE21_RS11410 2345715..2345930 - 216 WP_000066484.1 cold shock-like protein CspB -
FVE21_RS11415 2346231..2346443 + 213 WP_000087756.1 cold shock-like protein CspF -
FVE21_RS11420 2346498..2346587 + 90 WP_120795389.1 hypothetical protein -
FVE21_RS11425 2346865..2347617 - 753 WP_001047135.1 antitermination protein -
FVE21_RS11430 2347631..2348680 - 1050 WP_001393597.1 DUF968 domain-containing protein -
FVE21_RS11435 2348682..2348960 - 279 WP_012304870.1 hypothetical protein -
FVE21_RS11440 2349027..2349278 - 252 WP_000980994.1 hypothetical protein -
FVE21_RS11445 2349495..2349650 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FVE21_RS11450 2349722..2350009 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FVE21_RS11455 2350009..2350248 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FVE21_RS11460 2350273..2350578 + 306 WP_001326990.1 hypothetical protein -
FVE21_RS11465 2350781..2351113 + 333 WP_001301033.1 protein FlxA -
FVE21_RS11470 2351550..2351699 - 150 WP_011443592.1 hypothetical protein -
FVE21_RS11475 2351734..2352012 - 279 Protein_2204 hypothetical protein -
FVE21_RS11480 2351996..2352226 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FVE21_RS11485 2352310..2352717 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FVE21_RS11490 2352884..2353039 + 156 WP_000379575.1 DUF1391 family protein -
FVE21_RS11495 2353199..2353417 + 219 WP_001171942.1 hypothetical protein -
FVE21_RS11505 2353985..2354173 + 189 WP_000854559.1 cell division inhibition protein DicB -
FVE21_RS11510 2354170..2354361 + 192 WP_001083297.1 lysis protein YdfD -
FVE21_RS11515 2354454..2355221 + 768 Protein_2211 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2335378..2368839 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T136177 WP_000323025.1 NZ_CP042845:c2350009-2349722 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T136177 NZ_CP042845:c2350009-2349722 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT136177 WP_000534858.1 NZ_CP042845:c2350248-2350009 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT136177 NZ_CP055265:2260786-2261052 [Agrobacterium vitis]
ATGCTGGTCGCCTTTACAGATGAAGGCTGGGAAGATTACTGCTTCTGGCAGGAAACCGATCCCAAAACAGCGCAGCGGAT
CAATGCCCTGCTAAAGGACACCAAACGCCACCCCTTTTCAGGCATCGGCAAGCCAGAGCCACTGAAAGGCCAGTTCAAAG
GCTTCTGGTCACGCCGCATCAGCCAGGAACATCGGCTAGTTTATGCGGTGAAAGGTCAGGATGGCGATCAAGTGCTGATC
GTGGCGCAATGCCGCTATCATTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References