Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4101934..4102460 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GRF62_RS19665 Protein ID WP_000323025.1
Coordinates 4102173..4102460 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GRF62_RS19660 Protein ID WP_000534858.1
Coordinates 4101934..4102173 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS19600 4096961..4097728 - 768 Protein_3806 exonuclease -
GRF62_RS19605 4097821..4098012 - 192 WP_001083297.1 lysis protein YdfD -
GRF62_RS19610 4098009..4098197 - 189 WP_000854559.1 cell division inhibition protein DicB -
GRF62_RS19620 4098765..4098983 - 219 WP_001171942.1 hypothetical protein -
GRF62_RS19625 4099143..4099298 - 156 WP_000379575.1 DUF1391 family protein -
GRF62_RS19630 4099465..4099872 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
GRF62_RS19635 4099956..4100186 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
GRF62_RS19640 4100170..4100448 + 279 Protein_3813 hypothetical protein -
GRF62_RS19645 4100483..4100632 + 150 WP_011443592.1 hypothetical protein -
GRF62_RS19650 4101069..4101401 - 333 WP_001301033.1 protein FlxA -
GRF62_RS19655 4101604..4101909 - 306 WP_001326990.1 hypothetical protein -
GRF62_RS19660 4101934..4102173 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GRF62_RS19665 4102173..4102460 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GRF62_RS19670 4102532..4102687 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GRF62_RS19675 4102904..4103155 + 252 WP_000980994.1 hypothetical protein -
GRF62_RS19680 4103222..4103500 + 279 WP_012304870.1 hypothetical protein -
GRF62_RS19685 4103502..4104551 + 1050 WP_001265198.1 DUF968 domain-containing protein -
GRF62_RS19690 4104565..4105317 + 753 WP_001047135.1 antitermination protein -
GRF62_RS19695 4105595..4105684 - 90 WP_120795389.1 hypothetical protein -
GRF62_RS19700 4105739..4105951 - 213 WP_000087756.1 cold shock-like protein CspF -
GRF62_RS19705 4106252..4106467 + 216 WP_000066484.1 cold shock-like protein CspB -
GRF62_RS19710 4107221..4107436 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4091062..4119454 28392


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T145467 WP_000323025.1 NZ_CP047127:4102173-4102460 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T145467 NZ_CP062390:18899-19075 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT145467 WP_000534858.1 NZ_CP047127:4101934-4102173 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT145467 NZ_CP062390:c19237-19063 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References