145467

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4101934..4102460 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GRF62_RS19665 Protein ID WP_000323025.1
Coordinates 4102173..4102460 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GRF62_RS19660 Protein ID WP_000534858.1
Coordinates 4101934..4102173 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS19600 4096961..4097728 - 768 Protein_3806 exonuclease -
GRF62_RS19605 4097821..4098012 - 192 WP_001083297.1 lysis protein YdfD -
GRF62_RS19610 4098009..4098197 - 189 WP_000854559.1 cell division inhibition protein DicB -
GRF62_RS19620 4098765..4098983 - 219 WP_001171942.1 hypothetical protein -
GRF62_RS19625 4099143..4099298 - 156 WP_000379575.1 DUF1391 family protein -
GRF62_RS19630 4099465..4099872 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
GRF62_RS19635 4099956..4100186 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
GRF62_RS19640 4100170..4100448 + 279 Protein_3813 hypothetical protein -
GRF62_RS19645 4100483..4100632 + 150 WP_011443592.1 hypothetical protein -
GRF62_RS19650 4101069..4101401 - 333 WP_001301033.1 protein FlxA -
GRF62_RS19655 4101604..4101909 - 306 WP_001326990.1 hypothetical protein -
GRF62_RS19660 4101934..4102173 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GRF62_RS19665 4102173..4102460 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GRF62_RS19670 4102532..4102687 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GRF62_RS19675 4102904..4103155 + 252 WP_000980994.1 hypothetical protein -
GRF62_RS19680 4103222..4103500 + 279 WP_012304870.1 hypothetical protein -
GRF62_RS19685 4103502..4104551 + 1050 WP_001265198.1 DUF968 domain-containing protein -
GRF62_RS19690 4104565..4105317 + 753 WP_001047135.1 antitermination protein -
GRF62_RS19695 4105595..4105684 - 90 WP_120795389.1 hypothetical protein -
GRF62_RS19700 4105739..4105951 - 213 WP_000087756.1 cold shock-like protein CspF -
GRF62_RS19705 4106252..4106467 + 216 WP_000066484.1 cold shock-like protein CspB -
GRF62_RS19710 4107221..4107436 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4091062..4119454 28392


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T145467 WP_000323025.1 NZ_CP047127:4102173-4102460 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T145467 NZ_CP062390:18899-19075 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT145467 WP_000534858.1 NZ_CP047127:4101934-4102173 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT145467 NZ_CP062390:c19237-19063 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References