Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639603..1640129 Replicon chromosome
Accession NZ_CP062244
Organism Escherichia coli strain AML003_ev02

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IHN51_RS08030 Protein ID WP_000323025.1
Coordinates 1639603..1639890 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IHN51_RS08035 Protein ID WP_000534858.1
Coordinates 1639890..1640129 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN51_RS07985 1634627..1634842 - 216 WP_000839590.1 phage lysis protein EssD -
IHN51_RS07990 1635596..1635811 - 216 WP_000066484.1 cold shock-like protein CspB -
IHN51_RS07995 1636112..1636324 + 213 WP_000087756.1 cold shock-like protein CspF -
IHN51_RS08000 1636379..1636468 + 90 WP_120795389.1 hypothetical protein -
IHN51_RS08005 1636746..1637498 - 753 WP_001047135.1 antitermination protein -
IHN51_RS08010 1637512..1638561 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IHN51_RS08015 1638563..1638841 - 279 WP_012304870.1 hypothetical protein -
IHN51_RS08020 1638908..1639159 - 252 WP_000980994.1 hypothetical protein -
IHN51_RS08025 1639376..1639531 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IHN51_RS08030 1639603..1639890 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IHN51_RS08035 1639890..1640129 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IHN51_RS08040 1640154..1640459 + 306 WP_001326990.1 hypothetical protein -
IHN51_RS08045 1640662..1640994 + 333 WP_001301033.1 protein FlxA -
IHN51_RS08050 1641431..1641580 - 150 WP_011443592.1 hypothetical protein -
IHN51_RS08055 1641615..1641893 - 279 Protein_1574 hypothetical protein -
IHN51_RS08060 1641877..1642107 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IHN51_RS08065 1642191..1642598 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IHN51_RS08070 1642765..1642920 + 156 WP_000379575.1 DUF1391 family protein -
IHN51_RS08075 1643080..1643298 + 219 WP_001171942.1 hypothetical protein -
IHN51_RS08080 1643866..1644054 + 189 WP_000854559.1 cell division inhibition protein DicB -
IHN51_RS08085 1644051..1644242 + 192 WP_001083297.1 lysis protein YdfD -
IHN51_RS08090 1644335..1645102 + 768 Protein_1581 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625259..1658720 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T173861 WP_000323025.1 NZ_CP062244:c1639890-1639603 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T173861 NZ_CP080362:354808-355155 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT173861 WP_000534858.1 NZ_CP062244:c1640129-1639890 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT173861 NZ_CP080362:355155-355448 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References