Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2262093..2262619 Replicon chromosome
Accession NZ_CP038857
Organism Escherichia coli strain PigCaeca_2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag E5285_RS10965 Protein ID WP_000323025.1
Coordinates 2262332..2262619 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag E5285_RS10960 Protein ID WP_000534858.1
Coordinates 2262093..2262332 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5285_RS10945 2259578..2260806 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
E5285_RS10950 2261228..2261560 - 333 WP_135927300.1 FlxA-like family protein -
E5285_RS10955 2261763..2262068 - 306 WP_001326990.1 hypothetical protein -
E5285_RS10960 2262093..2262332 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
E5285_RS10965 2262332..2262619 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
E5285_RS10970 2262691..2262846 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
E5285_RS10975 2263063..2263314 + 252 WP_000980994.1 hypothetical protein -
E5285_RS10980 2263381..2263659 + 279 WP_012304870.1 hypothetical protein -
E5285_RS10985 2263661..2264710 + 1050 WP_001265198.1 DUF968 domain-containing protein -
E5285_RS10990 2264724..2265476 + 753 WP_001047135.1 antitermination protein -
E5285_RS10995 2265754..2265843 - 90 WP_120795389.1 hypothetical protein -
E5285_RS11000 2265898..2266110 - 213 WP_000087756.1 cold shock-like protein CspF -
E5285_RS11005 2266411..2266626 + 216 WP_000066484.1 cold shock-like protein CspB -
E5285_RS24630 2266990..2267160 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
E5285_RS11010 2267380..2267595 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2238467..2279604 41137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T123269 WP_000323025.1 NZ_CP038857:2262332-2262619 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T123269 NZ_CP038857:2262332-2262619 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT123269 WP_000534858.1 NZ_CP038857:2262093-2262332 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT123269 NZ_CP038857:2262093-2262332 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References