123269

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2262093..2262619 Replicon chromosome
Accession NZ_CP038857
Organism Escherichia coli strain PigCaeca_2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag E5285_RS10965 Protein ID WP_000323025.1
Coordinates 2262332..2262619 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag E5285_RS10960 Protein ID WP_000534858.1
Coordinates 2262093..2262332 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5285_RS10945 2259578..2260806 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
E5285_RS10950 2261228..2261560 - 333 WP_135927300.1 FlxA-like family protein -
E5285_RS10955 2261763..2262068 - 306 WP_001326990.1 hypothetical protein -
E5285_RS10960 2262093..2262332 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
E5285_RS10965 2262332..2262619 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
E5285_RS10970 2262691..2262846 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
E5285_RS10975 2263063..2263314 + 252 WP_000980994.1 hypothetical protein -
E5285_RS10980 2263381..2263659 + 279 WP_012304870.1 hypothetical protein -
E5285_RS10985 2263661..2264710 + 1050 WP_001265198.1 DUF968 domain-containing protein -
E5285_RS10990 2264724..2265476 + 753 WP_001047135.1 antitermination protein -
E5285_RS10995 2265754..2265843 - 90 WP_120795389.1 hypothetical protein -
E5285_RS11000 2265898..2266110 - 213 WP_000087756.1 cold shock-like protein CspF -
E5285_RS11005 2266411..2266626 + 216 WP_000066484.1 cold shock-like protein CspB -
E5285_RS24630 2266990..2267160 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
E5285_RS11010 2267380..2267595 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2238467..2279604 41137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T123269 WP_000323025.1 NZ_CP038857:2262332-2262619 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T123269 NZ_CP038857:2262332-2262619 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT123269 WP_000534858.1 NZ_CP038857:2262093-2262332 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT123269 NZ_CP038857:2262093-2262332 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References