Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4218550..4219076 Replicon chromosome
Accession NZ_CP043197
Organism Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FYA12_RS20495 Protein ID WP_000323025.1
Coordinates 4218789..4219076 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FYA12_RS20490 Protein ID WP_000534858.1
Coordinates 4218550..4218789 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA12_RS20430 4213577..4214344 - 768 Protein_3959 exonuclease -
FYA12_RS20435 4214437..4214628 - 192 WP_001083297.1 lysis protein YdfD -
FYA12_RS20440 4214625..4214813 - 189 WP_000854559.1 cell division inhibition protein DicB -
FYA12_RS20450 4215381..4215599 - 219 WP_001171942.1 hypothetical protein -
FYA12_RS20455 4215759..4215914 - 156 WP_000379575.1 DUF1391 family protein -
FYA12_RS20460 4216081..4216488 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FYA12_RS20465 4216572..4216802 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
FYA12_RS20470 4216786..4217064 + 279 Protein_3966 hypothetical protein -
FYA12_RS20475 4217099..4217248 + 150 WP_011443592.1 hypothetical protein -
FYA12_RS20480 4217685..4218017 - 333 WP_001301033.1 protein FlxA -
FYA12_RS20485 4218220..4218525 - 306 WP_001326990.1 hypothetical protein -
FYA12_RS20490 4218550..4218789 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FYA12_RS20495 4218789..4219076 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FYA12_RS20500 4219148..4219303 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FYA12_RS20505 4219520..4219771 + 252 WP_000980994.1 hypothetical protein -
FYA12_RS20510 4219838..4220116 + 279 WP_012304870.1 hypothetical protein -
FYA12_RS20515 4220118..4221167 + 1050 WP_001265198.1 DUF968 domain-containing protein -
FYA12_RS20520 4221181..4221933 + 753 WP_001047135.1 antitermination protein -
FYA12_RS20525 4222211..4222300 - 90 WP_120795389.1 hypothetical protein -
FYA12_RS20530 4222355..4222567 - 213 WP_000087756.1 cold shock-like protein CspF -
FYA12_RS20535 4222868..4223083 + 216 WP_000066484.1 cold shock-like protein CspB -
FYA12_RS23050 4223447..4223617 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
FYA12_RS20540 4223837..4224052 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4199959..4240317 40358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T137642 WP_000323025.1 NZ_CP043197:4218789-4219076 [Escherichia coli O16:H48]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T137642 NZ_CP056855:c141346-141059 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT137642 WP_000534858.1 NZ_CP043197:4218550-4218789 [Escherichia coli O16:H48]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT137642 NZ_CP056855:c141585-141346 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References