285657

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1649000..1649526 Replicon chromosome
Accession NZ_LN832404
Organism Escherichia coli K-12 substr. AG100

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EK12AG100_RS08255 Protein ID WP_000323025.1
Coordinates 1649000..1649287 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EK12AG100_RS08260 Protein ID WP_000534858.1
Coordinates 1649287..1649526 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EK12AG100_RS08215 1644024..1644239 - 216 WP_000839590.1 phage lysis protein EssD -
EK12AG100_RS08220 1644993..1645208 - 216 WP_000066484.1 cold shock-like protein CspB -
EK12AG100_RS08225 1645509..1645721 + 213 WP_000087756.1 cold shock-like protein CspF -
EK12AG100_RS25645 1645776..1645865 + 90 WP_120795389.1 hypothetical protein -
EK12AG100_RS08230 1646143..1646895 - 753 WP_001047135.1 antitermination protein -
EK12AG100_RS08235 1646909..1647958 - 1050 WP_001265198.1 DUF968 domain-containing protein -
EK12AG100_RS08240 1647960..1648238 - 279 WP_012304870.1 hypothetical protein -
EK12AG100_RS08245 1648305..1648556 - 252 WP_000980994.1 hypothetical protein -
EK12AG100_RS08250 1648773..1648928 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EK12AG100_RS08255 1649000..1649287 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EK12AG100_RS08260 1649287..1649526 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EK12AG100_RS23830 1649551..1649856 + 306 WP_001326990.1 hypothetical protein -
EK12AG100_RS08265 1650059..1650391 + 333 WP_001301033.1 protein FlxA -
EK12AG100_RS23115 1650828..1650977 - 150 WP_011443592.1 hypothetical protein -
EK12AG100_RS08270 1651012..1651290 - 279 Protein_1579 hypothetical protein -
EK12AG100_RS08275 1651274..1651504 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
EK12AG100_RS08280 1651588..1651995 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
EK12AG100_RS08285 1652162..1652317 + 156 WP_000379575.1 DUF1391 family protein -
EK12AG100_RS08290 1652477..1652695 + 219 WP_001171942.1 hypothetical protein -
EK12AG100_RS08300 1653263..1653451 + 189 WP_000854559.1 cell division inhibition protein DicB -
EK12AG100_RS08305 1653448..1653639 + 192 WP_001083297.1 lysis protein YdfD -
EK12AG100_RS23845 1653732..1654499 + 768 Protein_1586 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1634656..1668117 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T285657 WP_000323025.1 NZ_LN832404:c1649287-1649000 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT285657 WP_000534858.1 NZ_LN832404:c1649526-1649287 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References