Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4238716..4239242 Replicon chromosome
Accession NZ_CP013952
Organism Escherichia coli strain HST04

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AWH44_RS21900 Protein ID WP_000323025.1
Coordinates 4238955..4239242 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AWH44_RS21895 Protein ID WP_000534858.1
Coordinates 4238716..4238955 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWH44_RS21830 4233743..4234510 - 768 Protein_3966 exonuclease -
AWH44_RS21835 4234603..4234794 - 192 WP_001083297.1 lysis protein YdfD -
AWH44_RS21840 4234791..4234979 - 189 WP_000854559.1 cell division inhibition protein DicB -
AWH44_RS21850 4235547..4235765 - 219 WP_001171942.1 hypothetical protein -
AWH44_RS21860 4235925..4236080 - 156 WP_000379575.1 DUF1391 family protein -
AWH44_RS21865 4236247..4236654 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
AWH44_RS21870 4236738..4236968 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
AWH44_RS21875 4236952..4237230 + 279 Protein_3973 hypothetical protein -
AWH44_RS21880 4237265..4237414 + 150 WP_011443592.1 hypothetical protein -
AWH44_RS21885 4237851..4238183 - 333 WP_001301033.1 protein FlxA -
AWH44_RS21890 4238386..4238691 - 306 WP_001326990.1 hypothetical protein -
AWH44_RS21895 4238716..4238955 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AWH44_RS21900 4238955..4239242 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AWH44_RS21905 4239314..4239469 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
AWH44_RS21910 4239686..4239937 + 252 WP_000980994.1 hypothetical protein -
AWH44_RS21915 4240004..4240282 + 279 WP_012304870.1 hypothetical protein -
AWH44_RS21920 4240284..4241333 + 1050 WP_001265198.1 DUF968 domain-containing protein -
AWH44_RS21925 4241347..4242099 + 753 WP_001047135.1 antitermination protein -
AWH44_RS23940 4242377..4242466 - 90 WP_120795389.1 hypothetical protein -
AWH44_RS21930 4242521..4242733 - 213 WP_000087756.1 cold shock-like protein CspF -
AWH44_RS21935 4243034..4243249 + 216 WP_000066484.1 cold shock-like protein CspB -
AWH44_RS24340 4243613..4243783 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
AWH44_RS21940 4244003..4244218 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4226718..4253586 26868


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T59671 WP_000323025.1 NZ_CP013952:4238955-4239242 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T59671 NZ_CP013952:4238955-4239242 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT59671 WP_000534858.1 NZ_CP013952:4238716-4238955 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT59671 NZ_CP013952:4238716-4238955 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References