Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2526863..2527389 Replicon chromosome
Accession NZ_CP043195
Organism Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FYA13_RS12180 Protein ID WP_000323025.1
Coordinates 2527102..2527389 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FYA13_RS12175 Protein ID WP_000534858.1
Coordinates 2526863..2527102 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA13_RS12115 2521890..2522657 - 768 Protein_2346 exonuclease -
FYA13_RS12120 2522750..2522941 - 192 WP_001083297.1 lysis protein YdfD -
FYA13_RS12125 2522938..2523126 - 189 WP_000854559.1 cell division inhibition protein DicB -
FYA13_RS12135 2523694..2523912 - 219 WP_001171942.1 hypothetical protein -
FYA13_RS12140 2524072..2524227 - 156 WP_000379575.1 DUF1391 family protein -
FYA13_RS12145 2524394..2524801 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FYA13_RS12150 2524885..2525115 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
FYA13_RS12155 2525099..2525377 + 279 Protein_2353 hypothetical protein -
FYA13_RS12160 2525412..2525561 + 150 WP_011443592.1 hypothetical protein -
FYA13_RS12165 2525998..2526330 - 333 WP_001301033.1 protein FlxA -
FYA13_RS12170 2526533..2526838 - 306 WP_001326990.1 hypothetical protein -
FYA13_RS12175 2526863..2527102 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FYA13_RS12180 2527102..2527389 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FYA13_RS12185 2527461..2527616 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FYA13_RS12190 2527833..2528084 + 252 WP_000980994.1 hypothetical protein -
FYA13_RS12195 2528151..2528429 + 279 WP_012304870.1 hypothetical protein -
FYA13_RS12200 2528431..2529480 + 1050 WP_001265198.1 DUF968 domain-containing protein -
FYA13_RS12205 2529494..2530246 + 753 WP_001047135.1 antitermination protein -
FYA13_RS12210 2530524..2530613 - 90 WP_120795389.1 hypothetical protein -
FYA13_RS12215 2530668..2530880 - 213 WP_000087756.1 cold shock-like protein CspF -
FYA13_RS12220 2531181..2531396 + 216 WP_000066484.1 cold shock-like protein CspB -
FYA13_RS23350 2531760..2531930 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
FYA13_RS12225 2532150..2532365 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2508272..2548630 40358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T137602 WP_000323025.1 NZ_CP043195:2527102-2527389 [Escherichia coli O16:H48]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T137602 NZ_CP043195:2527102-2527389 [Escherichia coli O16:H48]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT137602 WP_000534858.1 NZ_CP043195:2526863-2527102 [Escherichia coli O16:H48]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT137602 NZ_CP056839:c4730643-4730401 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References