219999

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1648691..1649217 Replicon chromosome
Accession NZ_CP084897
Organism Escherichia coli strain gb37

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LHV64_RS08130 Protein ID WP_000323025.1
Coordinates 1648691..1648978 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LHV64_RS08135 Protein ID WP_000534858.1
Coordinates 1648978..1649217 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV64_RS08080 (1643715) 1643715..1643930 - 216 WP_000839590.1 phage lysis protein EssD -
LHV64_RS08085 (1644150) 1644150..1644320 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
LHV64_RS08090 (1644684) 1644684..1644899 - 216 WP_000066484.1 cold shock-like protein CspB -
LHV64_RS08095 (1645200) 1645200..1645412 + 213 WP_000087756.1 cold shock-like protein CspF -
LHV64_RS08100 (1645467) 1645467..1645556 + 90 WP_120795389.1 hypothetical protein -
LHV64_RS08105 (1645834) 1645834..1646586 - 753 WP_001047135.1 antitermination protein -
LHV64_RS08110 (1646600) 1646600..1647649 - 1050 WP_001393597.1 DUF968 domain-containing protein -
LHV64_RS08115 (1647651) 1647651..1647929 - 279 WP_012304870.1 hypothetical protein -
LHV64_RS08120 (1647996) 1647996..1648247 - 252 WP_000980994.1 protein Rem -
LHV64_RS08125 (1648464) 1648464..1648619 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LHV64_RS08130 (1648691) 1648691..1648978 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LHV64_RS08135 (1648978) 1648978..1649217 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LHV64_RS08140 (1649242) 1649242..1649547 + 306 WP_001326990.1 protein YdfV -
LHV64_RS08145 (1649750) 1649750..1650082 + 333 WP_001301033.1 FlxA-like family protein -
LHV64_RS08150 (1650519) 1650519..1650668 - 150 WP_011443592.1 protein YdfW -
LHV64_RS08155 (1650703) 1650703..1650981 - 279 Protein_1594 hypothetical protein -
LHV64_RS08160 (1650965) 1650965..1651195 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
LHV64_RS08165 (1651279) 1651279..1651686 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
LHV64_RS08170 (1651853) 1651853..1652008 + 156 WP_000379575.1 DUF1391 family protein -
LHV64_RS22615 (1652010) 1652010..1652138 + 129 WP_000344964.1 protein YdfB -
LHV64_RS08175 (1652168) 1652168..1652386 + 219 WP_001171942.1 protein YdfC -
LHV64_RS08180 (1652954) 1652954..1653142 + 189 WP_000854559.1 cell division inhibition protein DicB -
LHV64_RS08185 (1653139) 1653139..1653330 + 192 WP_001083297.1 lysis protein YdfD -
LHV64_RS08190 (1653423) 1653423..1654190 + 768 Protein_1602 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1634347..1660089 25742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T219999 WP_000323025.1 NZ_CP084897:c1648978-1648691 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T219999 NZ_CP116913:c4160436-4160263 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT219999 WP_000534858.1 NZ_CP084897:c1649217-1648978 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT219999 NZ_CP116913:c4160760-4160464 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATATTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References