Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639604..1640130 Replicon chromosome
Accession NZ_CP062248
Organism Escherichia coli strain AML002_ev02

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IHN47_RS08030 Protein ID WP_000323025.1
Coordinates 1639604..1639891 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IHN47_RS08035 Protein ID WP_000534858.1
Coordinates 1639891..1640130 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN47_RS07985 1634628..1634843 - 216 WP_000839590.1 phage lysis protein EssD -
IHN47_RS07990 1635597..1635812 - 216 WP_000066484.1 cold shock-like protein CspB -
IHN47_RS07995 1636113..1636325 + 213 WP_000087756.1 cold shock-like protein CspF -
IHN47_RS08000 1636380..1636469 + 90 WP_120795389.1 hypothetical protein -
IHN47_RS08005 1636747..1637499 - 753 WP_001047135.1 antitermination protein -
IHN47_RS08010 1637513..1638562 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IHN47_RS08015 1638564..1638842 - 279 WP_012304870.1 hypothetical protein -
IHN47_RS08020 1638909..1639160 - 252 WP_000980994.1 hypothetical protein -
IHN47_RS08025 1639377..1639532 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IHN47_RS08030 1639604..1639891 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IHN47_RS08035 1639891..1640130 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IHN47_RS08040 1640155..1640460 + 306 WP_001326990.1 hypothetical protein -
IHN47_RS08045 1640663..1640995 + 333 WP_001301033.1 protein FlxA -
IHN47_RS08050 1641432..1641581 - 150 WP_011443592.1 hypothetical protein -
IHN47_RS08055 1641616..1641894 - 279 Protein_1574 hypothetical protein -
IHN47_RS08060 1641878..1642108 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IHN47_RS08065 1642192..1642599 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IHN47_RS08070 1642766..1642921 + 156 WP_000379575.1 DUF1391 family protein -
IHN47_RS08075 1643081..1643299 + 219 WP_001171942.1 hypothetical protein -
IHN47_RS08080 1643867..1644055 + 189 WP_000854559.1 cell division inhibition protein DicB -
IHN47_RS08085 1644052..1644243 + 192 WP_001083297.1 lysis protein YdfD -
IHN47_RS08090 1644336..1645103 + 768 Protein_1581 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625260..1652128 26868


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T174033 WP_000323025.1 NZ_CP062248:c1639891-1639604 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T174033 NZ_CP080511:c3490858-3490280 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAGCGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCGCTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGGTGCCTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT174033 WP_000534858.1 NZ_CP062248:c1640130-1639891 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT174033 NZ_CP080511:c3491500-3490946 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References