Detailed information of TA system TA060779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3688239..3688765
• Replicon: chromosome
• Accession: NZ_CP014348
• Organism: Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: AWN69_RS18210
• Protein ID: WP_000323025.1
• Coordinates: 3688478..3688765 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: AWN69_RS18205
• Protein ID: WP_000534858.1
• Coordinates: 3688239..3688478 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWN69_RS24575	3683266..3684033	-	768	Protein_3454	exonuclease	-
AWN69_RS18155	3684126..3684317	-	192	WP_001083297.1	lysis protein YdfD	-
AWN69_RS18160	3684314..3684502	-	189	WP_000854559.1	cell division inhibition protein DicB	-
AWN69_RS18170	3685070..3685288	-	219	WP_001171942.1	hypothetical protein	-
AWN69_RS18175	3685448..3685603	-	156	WP_000379575.1	DUF1391 family protein	-
AWN69_RS18180	3685770..3686177	-	408	WP_000448564.1	DNA-binding transcriptional dual regulator DicA	-
AWN69_RS18185	3686261..3686491	+	231	WP_000920568.1	dicB transcriptional regulator DicC	-
AWN69_RS18190	3686475..3686753	+	279	Protein_3461	hypothetical protein	-
AWN69_RS18195	3686788..3686937	+	150	WP_011443592.1	hypothetical protein	-
AWN69_RS18200	3687374..3687706	-	333	WP_001301033.1	protein FlxA	-
AWN69_RS24590	3687909..3688214	-	306	WP_001326990.1	hypothetical protein	-
AWN69_RS18205	3688239..3688478	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
AWN69_RS18210	3688478..3688765	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
AWN69_RS18215	3688837..3688992	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
AWN69_RS18220	3689209..3689460	+	252	WP_000980994.1	hypothetical protein	-
AWN69_RS18225	3689527..3689805	+	279	WP_012304870.1	hypothetical protein	-
AWN69_RS18230	3689807..3690856	+	1050	WP_001393597.1	DUF968 domain-containing protein	-
AWN69_RS18235	3690870..3691622	+	753	WP_001047135.1	antitermination protein	-
AWN69_RS25880	3691900..3691989	-	90	WP_120795389.1	hypothetical protein	-
AWN69_RS18240	3692044..3692256	-	213	WP_000087756.1	cold shock-like protein CspF	-
AWN69_RS18245	3692557..3692772	+	216	WP_000066484.1	cold shock-like protein CspB	-
AWN69_RS26280	3693136..3693306	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
AWN69_RS18250	3693526..3693741	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Prophage TA020067 1519531..1552992 0.004 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Prophage TA001041 1631002..1664463 0.004 View NC_010473 Escherichia coli str. K-12 substr. DH10B Prophage TA022686 1718651..1753448 0.005 View NC_012759 Escherichia coli BW2952 Prophage TA023854 1521085..1554546 0.004 View NZ_CP009273 Escherichia coli BW25113 Prophage TA048683 1625259..1658720 0.004 View NZ_CP020543 Escherichia coli C Prophage TA075071 2249542..2301270 0.009 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Prophage TA285017 1519531..1552992 0.004	inside	Prophage	-	-	3669648..3710006	40358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T60779 WP_000323025.1 NZ_CP014348:3688478-3688765 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T60779 NZ_CP014348:3688478-3688765 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT60779 WP_000534858.1 NZ_CP014348:3688239-3688478 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT60779 NZ_CP014348:3688239-3688478 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8