Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3688239..3688765 Replicon chromosome
Accession NZ_CP014348
Organism Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AWN69_RS18210 Protein ID WP_000323025.1
Coordinates 3688478..3688765 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AWN69_RS18205 Protein ID WP_000534858.1
Coordinates 3688239..3688478 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN69_RS24575 3683266..3684033 - 768 Protein_3454 exonuclease -
AWN69_RS18155 3684126..3684317 - 192 WP_001083297.1 lysis protein YdfD -
AWN69_RS18160 3684314..3684502 - 189 WP_000854559.1 cell division inhibition protein DicB -
AWN69_RS18170 3685070..3685288 - 219 WP_001171942.1 hypothetical protein -
AWN69_RS18175 3685448..3685603 - 156 WP_000379575.1 DUF1391 family protein -
AWN69_RS18180 3685770..3686177 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
AWN69_RS18185 3686261..3686491 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
AWN69_RS18190 3686475..3686753 + 279 Protein_3461 hypothetical protein -
AWN69_RS18195 3686788..3686937 + 150 WP_011443592.1 hypothetical protein -
AWN69_RS18200 3687374..3687706 - 333 WP_001301033.1 protein FlxA -
AWN69_RS24590 3687909..3688214 - 306 WP_001326990.1 hypothetical protein -
AWN69_RS18205 3688239..3688478 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AWN69_RS18210 3688478..3688765 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AWN69_RS18215 3688837..3688992 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
AWN69_RS18220 3689209..3689460 + 252 WP_000980994.1 hypothetical protein -
AWN69_RS18225 3689527..3689805 + 279 WP_012304870.1 hypothetical protein -
AWN69_RS18230 3689807..3690856 + 1050 WP_001393597.1 DUF968 domain-containing protein -
AWN69_RS18235 3690870..3691622 + 753 WP_001047135.1 antitermination protein -
AWN69_RS25880 3691900..3691989 - 90 WP_120795389.1 hypothetical protein -
AWN69_RS18240 3692044..3692256 - 213 WP_000087756.1 cold shock-like protein CspF -
AWN69_RS18245 3692557..3692772 + 216 WP_000066484.1 cold shock-like protein CspB -
AWN69_RS26280 3693136..3693306 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
AWN69_RS18250 3693526..3693741 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3669648..3710006 40358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T60779 WP_000323025.1 NZ_CP014348:3688478-3688765 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T60779 NZ_CP014348:3688478-3688765 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT60779 WP_000534858.1 NZ_CP014348:3688239-3688478 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT60779 NZ_CP014348:3688239-3688478 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References