146827

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2249965..2250491 Replicon chromosome
Accession NZ_CP047594
Organism Escherichia coli strain LD27-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GUU88_RS10955 Protein ID WP_000323025.1
Coordinates 2249965..2250252 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GUU88_RS10960 Protein ID WP_000534858.1
Coordinates 2250252..2250491 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU88_RS10910 2245163..2245696 - 534 WP_001092971.1 lysozyme -
GUU88_RS10915 2245693..2246004 - 312 WP_000189916.1 YdfR family protein -
GUU88_RS10920 2246009..2246224 - 216 WP_000839590.1 phage lysis protein EssD -
GUU88_RS10925 2246978..2247193 - 216 WP_000066484.1 cold shock-like protein CspB -
GUU88_RS10930 2247494..2247706 + 213 WP_000087756.1 cold shock-like protein CspF -
GUU88_RS10935 2247761..2247850 + 90 WP_120795389.1 hypothetical protein -
GUU88_RS10940 2248128..2248880 - 753 WP_001047135.1 antitermination protein -
GUU88_RS10945 2248894..2249568 - 675 Protein_2137 DUF968 domain-containing protein -
GUU88_RS10950 2249738..2249893 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GUU88_RS10955 2249965..2250252 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GUU88_RS10960 2250252..2250491 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GUU88_RS10965 2250516..2250821 + 306 WP_001326990.1 hypothetical protein -
GUU88_RS10970 2251024..2251356 + 333 WP_001301033.1 protein FlxA -
GUU88_RS10975 2251793..2253106 - 1314 WP_000589005.1 ISNCY family transposase -
GUU88_RS22820 2254189..2254380 - 192 Protein_2144 hypothetical protein -
GUU88_RS10990 2254364..2254594 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
GUU88_RS10995 2254678..2255085 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
GUU88_RS11000 2255252..2255407 + 156 WP_000379575.1 DUF1391 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2235900..2267399 31499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T146827 WP_000323025.1 NZ_CP047594:c2250252-2249965 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T146827 NZ_CP062804:270503-270919 [Cupriavidus basilensis]
ATGTATTTGATTGACACCAATGTCATCAGTGAGATGAGGAAGCGCGAGCGTGCCGACAAGGGTGTTCGAGCATTCTTTCG
CCAAGCGGCAAAGCAGGAGAGTGAGCTGTACTTGTCGGTCGTCACTGTCGGCGAACTTCGGCGTGGCGTCGAGCTGATCC
AGCATCGGGGAGATCATGCGCAAGGCGCGCTTCTTGCCACGTGGCTCGACACGGTGCTGCAAGAGTTCGGGGCACATATC
CTCGCGGTAGATGCGGAAATCGGTCAGCTATGGGGGCGCCTTCGTGTCCCCCACGCGGAGCATGCCTTGGACAAACTGAT
CGCGGCGACGGCGCTGATTCACGACCTCACCGTAGTGACGCGCAACGTTAGTGATTTTGCTGGTACCGGGGCGCGTGTTC
TGAACCCCTTTGAATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT146827 WP_000534858.1 NZ_CP047594:c2250491-2250252 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT146827 NZ_CP062804:270280-270516 [Cupriavidus basilensis]
ATGGCAAATCTGCTGGTTCGAGGGATTGATGAAGCGCTAGTACAGAGGCTGCGTGAGCAGGCTGCTGCACACGGTCGTAG
CGCTGAGGCCGAGCACCGTGAAATTCTTGCGCGGGCGTTGCACACGACCAGGCGCAAGAGCTTTGCCGAGGTGTTGGCAA
GCATGCCAGATGTCGGCGAGGACAGCGATTTCGAGCGCGTCGATCAGCCGGGCGGGGCCGCGCATGTATTTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References