218255

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3042729..3043255 Replicon chromosome
Accession NZ_CP084207
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LDB15_RS14610 Protein ID WP_000323025.1
Coordinates 3042968..3043255 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LDB15_RS14605 Protein ID WP_000534858.1
Coordinates 3042729..3042968 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS14550 (3037756) 3037756..3038523 - 768 Protein_2839 exonuclease -
LDB15_RS14555 (3038616) 3038616..3038807 - 192 WP_001083297.1 lysis protein YdfD -
LDB15_RS14560 (3038804) 3038804..3038992 - 189 WP_000854559.1 cell division inhibition protein DicB -
LDB15_RS14565 (3039560) 3039560..3039778 - 219 WP_001171942.1 protein YdfC -
LDB15_RS23330 (3039808) 3039808..3039936 - 129 WP_000344964.1 protein YdfB -
LDB15_RS14570 (3039938) 3039938..3040093 - 156 WP_000379575.1 DUF1391 family protein -
LDB15_RS14575 (3040260) 3040260..3040667 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
LDB15_RS14580 (3040751) 3040751..3040981 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
LDB15_RS14585 (3040965) 3040965..3041243 + 279 Protein_2847 protein YdfX -
LDB15_RS14590 (3041278) 3041278..3041427 + 150 WP_011443592.1 protein YdfW -
LDB15_RS14595 (3041864) 3041864..3042196 - 333 WP_001301033.1 FlxA-like family protein -
LDB15_RS14600 (3042399) 3042399..3042704 - 306 WP_001326990.1 protein YdfV -
LDB15_RS14605 (3042729) 3042729..3042968 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LDB15_RS14610 (3042968) 3042968..3043255 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LDB15_RS14615 (3043327) 3043327..3043482 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LDB15_RS14620 (3043699) 3043699..3043950 + 252 WP_000980994.1 protein Rem -
LDB15_RS14625 (3044017) 3044017..3044295 + 279 WP_012304870.1 hypothetical protein -
LDB15_RS14630 (3044297) 3044297..3045346 + 1050 WP_001265198.1 DUF968 domain-containing protein -
LDB15_RS14635 (3045360) 3045360..3046112 + 753 WP_001047135.1 antitermination protein -
LDB15_RS14640 (3046390) 3046390..3046479 - 90 WP_120795389.1 hypothetical protein -
LDB15_RS14645 (3046534) 3046534..3046746 - 213 WP_000087756.1 cold shock-like protein CspF -
LDB15_RS14650 (3047047) 3047047..3047262 + 216 WP_000066484.1 cold shock-like protein CspB -
LDB15_RS14655 (3047626) 3047626..3047796 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
LDB15_RS14660 (3048016) 3048016..3048231 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3031857..3059386 27529


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T218255 WP_000323025.1 NZ_CP084207:3042968-3043255 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T218255 NZ_CP115712:c4384948-4384664 [Kosakonia pseudosacchari]
ATGAATTATGAGCTGGTGTTTGATCCCCGCGCATACAAAGAATGGAAAAAACTCGGTGCCACGGTAAAAACACAGTTCAA
AAAGAAACTGGCGGAGGTGCTGGTTCAACCCAGAATTGAAAAGGCCCGACTGCATCAATTTCCTGATTGCTACAAAATCA
AATTGCGAACATCAGGCTACCGGTTGGTCTATCAGGTCAGAGACAATGTGGTGACGGTATTCGTTGTGTCTATCGGTAAA
CGCGAAAAAGCCGCCGCTTATCAGGATGCCAGCAAGCGGCTCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT218255 WP_000534858.1 NZ_CP084207:3042729-3042968 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT218255 NZ_CP115712:c4385180-4384938 [Kosakonia pseudosacchari]
ATGGCGACGTTAAATGTTCGACTGGATGACAAACTCAAAGAAGAGGCCTGGCGCGTGCTGGAAAAACTCAATATCACCCC
AACCGAAGCCGTTCGCTTACTGTTTCAATATGTCGCTGAAAACGGGCGTATGCCGATCAAAACAGTGACGGTGAGCGAGG
ACGAATCGGCGTTGCTGGAGATTGTCCGGGAGCGGCTGGCGAACCCGCAAAAAGGCATCAAGGTTAATCTGGATGAATTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References