Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3228204..3228730 Replicon chromosome
Accession NZ_CP011124
Organism Escherichia coli strain USML2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag USML2_RS16425 Protein ID WP_000323025.1
Coordinates 3228443..3228730 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag USML2_RS16420 Protein ID WP_000534858.1
Coordinates 3228204..3228443 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
USML2_RS16355 3223231..3223998 - 768 Protein_2986 exonuclease -
USML2_RS16360 3224091..3224282 - 192 WP_001083297.1 lysis protein YdfD -
USML2_RS16365 3224279..3224467 - 189 WP_000854559.1 cell division inhibition protein DicB -
USML2_RS16375 3225035..3225253 - 219 WP_001171942.1 hypothetical protein -
USML2_RS16385 3225413..3225568 - 156 WP_000379575.1 DUF1391 family protein -
USML2_RS16390 3225735..3226142 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
USML2_RS16395 3226226..3226456 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
USML2_RS16400 3226440..3226718 + 279 Protein_2993 hypothetical protein -
USML2_RS16405 3226753..3226902 + 150 WP_011443592.1 hypothetical protein -
USML2_RS16410 3227339..3227671 - 333 WP_001301033.1 protein FlxA -
USML2_RS16415 3227874..3228179 - 306 WP_001326990.1 hypothetical protein -
USML2_RS16420 3228204..3228443 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
USML2_RS16425 3228443..3228730 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
USML2_RS16430 3228802..3228957 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
USML2_RS16435 3229174..3229425 + 252 WP_000980994.1 hypothetical protein -
USML2_RS16440 3229492..3229770 + 279 WP_012304870.1 hypothetical protein -
USML2_RS16445 3229772..3230821 + 1050 WP_001265198.1 DUF968 domain-containing protein -
USML2_RS16450 3230835..3231587 + 753 WP_001047135.1 antitermination protein -
USML2_RS23280 3231865..3231954 - 90 WP_120795389.1 hypothetical protein -
USML2_RS16455 3232009..3232221 - 213 WP_000087756.1 cold shock-like protein CspF -
USML2_RS16460 3232522..3232737 + 216 WP_000066484.1 cold shock-like protein CspB -
USML2_RS23600 3233101..3233271 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
USML2_RS16465 3233491..3233706 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3209613..3249971 40358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T53503 WP_000323025.1 NZ_CP011124:3228443-3228730 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T53503 NZ_CP011124:3228443-3228730 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT53503 WP_000534858.1 NZ_CP011124:3228204-3228443 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT53503 NZ_CP011124:3228204-3228443 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References