53503

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3228204..3228730 Replicon chromosome
Accession NZ_CP011124
Organism Escherichia coli strain USML2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag USML2_RS16425 Protein ID WP_000323025.1
Coordinates 3228443..3228730 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag USML2_RS16420 Protein ID WP_000534858.1
Coordinates 3228204..3228443 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
USML2_RS16355 3223231..3223998 - 768 Protein_2986 exonuclease -
USML2_RS16360 3224091..3224282 - 192 WP_001083297.1 lysis protein YdfD -
USML2_RS16365 3224279..3224467 - 189 WP_000854559.1 cell division inhibition protein DicB -
USML2_RS16375 3225035..3225253 - 219 WP_001171942.1 hypothetical protein -
USML2_RS16385 3225413..3225568 - 156 WP_000379575.1 DUF1391 family protein -
USML2_RS16390 3225735..3226142 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
USML2_RS16395 3226226..3226456 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
USML2_RS16400 3226440..3226718 + 279 Protein_2993 hypothetical protein -
USML2_RS16405 3226753..3226902 + 150 WP_011443592.1 hypothetical protein -
USML2_RS16410 3227339..3227671 - 333 WP_001301033.1 protein FlxA -
USML2_RS16415 3227874..3228179 - 306 WP_001326990.1 hypothetical protein -
USML2_RS16420 3228204..3228443 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
USML2_RS16425 3228443..3228730 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
USML2_RS16430 3228802..3228957 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
USML2_RS16435 3229174..3229425 + 252 WP_000980994.1 hypothetical protein -
USML2_RS16440 3229492..3229770 + 279 WP_012304870.1 hypothetical protein -
USML2_RS16445 3229772..3230821 + 1050 WP_001265198.1 DUF968 domain-containing protein -
USML2_RS16450 3230835..3231587 + 753 WP_001047135.1 antitermination protein -
USML2_RS23280 3231865..3231954 - 90 WP_120795389.1 hypothetical protein -
USML2_RS16455 3232009..3232221 - 213 WP_000087756.1 cold shock-like protein CspF -
USML2_RS16460 3232522..3232737 + 216 WP_000066484.1 cold shock-like protein CspB -
USML2_RS23600 3233101..3233271 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
USML2_RS16465 3233491..3233706 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3209613..3249971 40358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T53503 WP_000323025.1 NZ_CP011124:3228443-3228730 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T53503 NZ_CP011124:3228443-3228730 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT53503 WP_000534858.1 NZ_CP011124:3228204-3228443 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT53503 NZ_CP011124:3228204-3228443 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References