120213

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639265..1639791 Replicon chromosome
Accession NZ_CP037857
Organism Escherichia coli BW25113 strain AM187

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag E1A98_RS08085 Protein ID WP_000323025.1
Coordinates 1639265..1639552 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag E1A98_RS08090 Protein ID WP_000534858.1
Coordinates 1639552..1639791 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1A98_RS08040 1634289..1634504 - 216 WP_000839590.1 phage lysis protein EssD -
E1A98_RS22670 1634724..1634894 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
E1A98_RS08045 1635258..1635473 - 216 WP_000066484.1 cold shock-like protein CspB -
E1A98_RS08050 1635774..1635986 + 213 WP_000087756.1 cold shock-like protein CspF -
E1A98_RS08055 1636041..1636130 + 90 WP_120795389.1 hypothetical protein -
E1A98_RS08060 1636408..1637160 - 753 WP_001047135.1 antitermination protein -
E1A98_RS08065 1637174..1638223 - 1050 WP_001393597.1 DUF968 domain-containing protein -
E1A98_RS08070 1638225..1638503 - 279 WP_012304870.1 hypothetical protein -
E1A98_RS08075 1638570..1638821 - 252 WP_000980994.1 hypothetical protein -
E1A98_RS08080 1639038..1639193 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
E1A98_RS08085 1639265..1639552 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
E1A98_RS08090 1639552..1639791 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
E1A98_RS08095 1639816..1640121 + 306 WP_001326990.1 hypothetical protein -
E1A98_RS08100 1640324..1640656 + 333 WP_001301033.1 protein FlxA -
E1A98_RS08105 1641093..1641242 - 150 WP_011443592.1 hypothetical protein -
E1A98_RS08110 1641277..1641555 - 279 Protein_1580 hypothetical protein -
E1A98_RS08115 1641539..1641769 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
E1A98_RS08120 1641853..1642260 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
E1A98_RS08125 1642427..1642582 + 156 WP_000379575.1 DUF1391 family protein -
E1A98_RS08130 1642742..1642960 + 219 WP_001171942.1 hypothetical protein -
E1A98_RS08140 1643528..1643716 + 189 WP_000854559.1 cell division inhibition protein DicB -
E1A98_RS08145 1643713..1643904 + 192 WP_001083297.1 lysis protein YdfD -
E1A98_RS08150 1643997..1644764 + 768 Protein_1587 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1624921..1658382 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T120213 WP_000323025.1 NZ_CP037857:c1639552-1639265 [Escherichia coli BW25113]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T120213 NZ_CP037857:c1639552-1639265 [Escherichia coli BW25113]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT120213 WP_000534858.1 NZ_CP037857:c1639791-1639552 [Escherichia coli BW25113]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT120213 NZ_CP037857:c1639791-1639552 [Escherichia coli BW25113]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References