Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1639265..1639791 Replicon chromosome
Accession NZ_CP037857
Organism Escherichia coli BW25113 strain AM187

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag E1A98_RS08085 Protein ID WP_000323025.1
Coordinates 1639265..1639552 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag E1A98_RS08090 Protein ID WP_000534858.1
Coordinates 1639552..1639791 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1A98_RS08040 1634289..1634504 - 216 WP_000839590.1 phage lysis protein EssD -
E1A98_RS22670 1634724..1634894 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
E1A98_RS08045 1635258..1635473 - 216 WP_000066484.1 cold shock-like protein CspB -
E1A98_RS08050 1635774..1635986 + 213 WP_000087756.1 cold shock-like protein CspF -
E1A98_RS08055 1636041..1636130 + 90 WP_120795389.1 hypothetical protein -
E1A98_RS08060 1636408..1637160 - 753 WP_001047135.1 antitermination protein -
E1A98_RS08065 1637174..1638223 - 1050 WP_001393597.1 DUF968 domain-containing protein -
E1A98_RS08070 1638225..1638503 - 279 WP_012304870.1 hypothetical protein -
E1A98_RS08075 1638570..1638821 - 252 WP_000980994.1 hypothetical protein -
E1A98_RS08080 1639038..1639193 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
E1A98_RS08085 1639265..1639552 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
E1A98_RS08090 1639552..1639791 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
E1A98_RS08095 1639816..1640121 + 306 WP_001326990.1 hypothetical protein -
E1A98_RS08100 1640324..1640656 + 333 WP_001301033.1 protein FlxA -
E1A98_RS08105 1641093..1641242 - 150 WP_011443592.1 hypothetical protein -
E1A98_RS08110 1641277..1641555 - 279 Protein_1580 hypothetical protein -
E1A98_RS08115 1641539..1641769 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
E1A98_RS08120 1641853..1642260 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
E1A98_RS08125 1642427..1642582 + 156 WP_000379575.1 DUF1391 family protein -
E1A98_RS08130 1642742..1642960 + 219 WP_001171942.1 hypothetical protein -
E1A98_RS08140 1643528..1643716 + 189 WP_000854559.1 cell division inhibition protein DicB -
E1A98_RS08145 1643713..1643904 + 192 WP_001083297.1 lysis protein YdfD -
E1A98_RS08150 1643997..1644764 + 768 Protein_1587 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1624921..1658382 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T120213 WP_000323025.1 NZ_CP037857:c1639552-1639265 [Escherichia coli BW25113]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T120213 NZ_CP037857:c1639552-1639265 [Escherichia coli BW25113]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT120213 WP_000534858.1 NZ_CP037857:c1639791-1639552 [Escherichia coli BW25113]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT120213 NZ_CP037857:c1639791-1639552 [Escherichia coli BW25113]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References