Detailed information of TA system TA033893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2478780..2479306
• Replicon: chromosome
• Accession: NZ_AP021946
• Organism: Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: H7R07_RS12070
• Protein ID: WP_000323025.1
• Coordinates: 2479019..2479306 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: H7R07_RS12065
• Protein ID: WP_000534858.1
• Coordinates: 2478780..2479019 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R07_RS12040	2474139..2474486	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R07_RS12045	2474483..2474887	-	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
H7R07_RS26620	2475109..2475396	+	288	Protein_2363	hypothetical protein	-
H7R07_RS12050	2476165..2477478	+	1314	WP_000589005.1	ISNCY family transposase	-
H7R07_RS12055	2477915..2478247	-	333	WP_001301033.1	protein FlxA	-
H7R07_RS12060	2478450..2478755	-	306	WP_001326990.1	hypothetical protein	-
H7R07_RS12065	2478780..2479019	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
H7R07_RS12070	2479019..2479306	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
H7R07_RS12075	2479378..2479533	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
H7R07_RS12080	2479750..2480001	+	252	WP_000980994.1	hypothetical protein	-
H7R07_RS12085	2480068..2480346	+	279	WP_012304870.1	hypothetical protein	-
H7R07_RS12090	2480348..2481397	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
H7R07_RS12095	2481411..2482163	+	753	WP_001047135.1	antitermination protein	-
H7R07_RS12100	2482441..2482530	-	90	WP_120795389.1	hypothetical protein	-
H7R07_RS12105	2482585..2482797	-	213	WP_000087756.1	cold shock-like protein CspF	-
H7R07_RS12110	2483098..2483313	+	216	WP_000066484.1	cold shock-like protein CspB	-
H7R07_RS12115	2484067..2484282	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Prophage TA020067 1519531..1552992 0.008 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Prophage TA001041 1631002..1664463 0.008 View NC_010473 Escherichia coli str. K-12 substr. DH10B Prophage TA022686 1718651..1753448 0.008 View NC_012759 Escherichia coli BW2952 Prophage TA023854 1521085..1554546 0.008 View NZ_CP009273 Escherichia coli BW25113 Prophage TA048683 1625259..1658720 0.008 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Prophage TA285017 1519531..1552992 0.008	inside	Prophage	-	-	2451962..2495155	43193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T33893 WP_000323025.1 NZ_AP021946:2479019-2479306 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T33893 NZ_AP021946:2479019-2479306 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT33893 WP_000534858.1 NZ_AP021946:2478780-2479019 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT33893 NZ_AP021946:2478780-2479019 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8