Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2478780..2479306 Replicon chromosome
Accession NZ_AP021946
Organism Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R07_RS12070 Protein ID WP_000323025.1
Coordinates 2479019..2479306 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R07_RS12065 Protein ID WP_000534858.1
Coordinates 2478780..2479019 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R07_RS12040 2474139..2474486 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
H7R07_RS12045 2474483..2474887 - 405 WP_000839179.1 IS66 family insertion sequence hypothetical protein -
H7R07_RS26620 2475109..2475396 + 288 Protein_2363 hypothetical protein -
H7R07_RS12050 2476165..2477478 + 1314 WP_000589005.1 ISNCY family transposase -
H7R07_RS12055 2477915..2478247 - 333 WP_001301033.1 protein FlxA -
H7R07_RS12060 2478450..2478755 - 306 WP_001326990.1 hypothetical protein -
H7R07_RS12065 2478780..2479019 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R07_RS12070 2479019..2479306 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R07_RS12075 2479378..2479533 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
H7R07_RS12080 2479750..2480001 + 252 WP_000980994.1 hypothetical protein -
H7R07_RS12085 2480068..2480346 + 279 WP_012304870.1 hypothetical protein -
H7R07_RS12090 2480348..2481397 + 1050 WP_001265199.1 DUF968 domain-containing protein -
H7R07_RS12095 2481411..2482163 + 753 WP_001047135.1 antitermination protein -
H7R07_RS12100 2482441..2482530 - 90 WP_120795389.1 hypothetical protein -
H7R07_RS12105 2482585..2482797 - 213 WP_000087756.1 cold shock-like protein CspF -
H7R07_RS12110 2483098..2483313 + 216 WP_000066484.1 cold shock-like protein CspB -
H7R07_RS12115 2484067..2484282 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2451962..2495155 43193


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T33893 WP_000323025.1 NZ_AP021946:2479019-2479306 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T33893 NZ_AP021946:2479019-2479306 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT33893 WP_000534858.1 NZ_AP021946:2478780-2479019 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT33893 NZ_AP021946:2478780-2479019 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References