210527

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2499974..2500500 Replicon chromosome
Accession NZ_CP079993
Organism Escherichia coli strain EV36

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KYK84_RS12140 Protein ID WP_000323025.1
Coordinates 2499974..2500261 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KYK84_RS12145 Protein ID WP_000534858.1
Coordinates 2500261..2500500 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK84_RS12090 (2494998) 2494998..2495213 - 216 WP_000839590.1 phage lysis protein EssD -
KYK84_RS12095 (2495433) 2495433..2495603 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
KYK84_RS12100 (2495967) 2495967..2496182 - 216 WP_000066484.1 cold shock-like protein CspB -
KYK84_RS12105 (2496483) 2496483..2496695 + 213 WP_000087756.1 cold shock-like protein CspF -
KYK84_RS12110 (2496750) 2496750..2496839 + 90 WP_120795389.1 hypothetical protein -
KYK84_RS12115 (2497117) 2497117..2497869 - 753 WP_001047135.1 antitermination protein -
KYK84_RS12120 (2497883) 2497883..2498932 - 1050 WP_001393597.1 DUF968 domain-containing protein -
KYK84_RS12125 (2498934) 2498934..2499212 - 279 WP_012304870.1 hypothetical protein -
KYK84_RS12130 (2499279) 2499279..2499530 - 252 WP_000980994.1 protein Rem -
KYK84_RS12135 (2499747) 2499747..2499902 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
KYK84_RS12140 (2499974) 2499974..2500261 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KYK84_RS12145 (2500261) 2500261..2500500 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KYK84_RS12150 (2500525) 2500525..2500830 + 306 WP_001326990.1 protein YdfV -
KYK84_RS12155 (2501033) 2501033..2501365 + 333 WP_001301033.1 FlxA-like family protein -
KYK84_RS12160 (2501802) 2501802..2501951 - 150 WP_011443592.1 protein YdfW -
KYK84_RS12165 (2501986) 2501986..2502264 - 279 Protein_2389 protein YdfX -
KYK84_RS12170 (2502248) 2502248..2502478 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
KYK84_RS12175 (2502562) 2502562..2502969 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
KYK84_RS12180 (2503136) 2503136..2503291 + 156 WP_000379575.1 DUF1391 family protein -
KYK84_RS22925 (2503293) 2503293..2503421 + 129 WP_000344964.1 protein YdfB -
KYK84_RS12185 (2503451) 2503451..2503669 + 219 WP_001171942.1 protein YdfC -
KYK84_RS12190 (2504237) 2504237..2504425 + 189 WP_000854559.1 cell division inhibition protein DicB -
KYK84_RS12195 (2504422) 2504422..2504613 + 192 WP_001083297.1 lysis protein YdfD -
KYK84_RS12200 (2504706) 2504706..2505473 + 768 Protein_2397 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2485630..2519091 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T210527 WP_000323025.1 NZ_CP079993:c2500261-2499974 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T210527 NZ_CP104536:1082150-1082461 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT210527 WP_000534858.1 NZ_CP079993:c2500500-2500261 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT210527 NZ_CP104536:1082458-1082877 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References