Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2995800..2996326 Replicon chromosome
Accession NZ_CP012869
Organism Escherichia coli strain K-12 substr. MG1655_TMP32XR1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag QR62_RS14735 Protein ID WP_000323025.1
Coordinates 2996039..2996326 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag QR62_RS14730 Protein ID WP_000534858.1
Coordinates 2995800..2996039 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QR62_RS24125 2990827..2991594 - 768 Protein_2777 exonuclease -
QR62_RS14685 2991687..2991878 - 192 WP_001083297.1 lysis protein YdfD -
QR62_RS14690 2991875..2992063 - 189 WP_000854559.1 cell division inhibition protein DicB -
QR62_RS14700 2992631..2992849 - 219 WP_001171942.1 hypothetical protein -
QR62_RS14705 2993009..2993164 - 156 WP_000379575.1 DUF1391 family protein -
QR62_RS14710 2993331..2993738 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
QR62_RS14715 2993822..2994052 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
QR62_RS14720 2994036..2994314 + 279 Protein_2784 hypothetical protein -
QR62_RS22955 2994349..2994498 + 150 WP_011443592.1 hypothetical protein -
QR62_RS14725 2994935..2995267 - 333 WP_001301033.1 protein FlxA -
QR62_RS24140 2995470..2995775 - 306 WP_001326990.1 hypothetical protein -
QR62_RS14730 2995800..2996039 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
QR62_RS14735 2996039..2996326 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
QR62_RS14740 2996398..2996553 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
QR62_RS14745 2996770..2997021 + 252 WP_000980994.1 hypothetical protein -
QR62_RS14750 2997088..2997366 + 279 WP_012304870.1 hypothetical protein -
QR62_RS14755 2997368..2998417 + 1050 WP_001393597.1 DUF968 domain-containing protein -
QR62_RS14760 2998431..2999183 + 753 WP_001047135.1 antitermination protein -
QR62_RS25635 2999461..2999550 - 90 WP_120795389.1 hypothetical protein -
QR62_RS14765 2999605..2999817 - 213 WP_000087756.1 cold shock-like protein CspF -
QR62_RS14770 3000118..3000333 + 216 WP_000066484.1 cold shock-like protein CspB -
QR62_RS26160 3000697..3000867 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
QR62_RS14775 3001087..3001302 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2977209..3010670 33461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T57339 WP_000323025.1 NZ_CP012869:2996039-2996326 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T57339 NZ_CP012869:2996039-2996326 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT57339 WP_000534858.1 NZ_CP012869:2995800-2996039 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT57339 NZ_CP012869:2995800-2996039 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References